中国食品学报2024,Vol.24Issue(9) :310-321.DOI:10.16429/j.1009-7848.2024.09.029

不同乳酸菌发酵对雪花梨汁营养成分、抗氧化活性及挥发性风味物质的影响

Effects of Different Lactic Acid Bacteria Fermentation on Nutrient Composition,Antioxidant Activity and Volatile Flavor Substances of Snow Pear Juice

吴江娜 魏冠棉 毛娜 田桂芳 高洁 李福宽 桑亚新
中国食品学报2024,Vol.24Issue(9) :310-321.DOI:10.16429/j.1009-7848.2024.09.029

不同乳酸菌发酵对雪花梨汁营养成分、抗氧化活性及挥发性风味物质的影响

Effects of Different Lactic Acid Bacteria Fermentation on Nutrient Composition,Antioxidant Activity and Volatile Flavor Substances of Snow Pear Juice

吴江娜 1魏冠棉 1毛娜 1田桂芳 1高洁 1李福宽 2桑亚新1
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作者信息

  • 1. 河北农业大学食品科技学院河北保定 071000
  • 2. 张家口市崇礼区市场监督管理局 河北张家口 076350
  • 折叠

摘要

为提升雪花梨汁的抗氧化活性和风味,分别以干酪乳杆菌Y5-2b、植物乳杆菌C17和嗜酸乳杆菌CH-2制备发酵梨汁,对其理化指标和抗氧化活性进行分析,并采用高效液相色谱法和气相离子迁移谱技术(GC-IMS)对其营养物质和香气成分进行研究.结果表明:3株乳酸菌均能在梨汁中进行良好代谢,且Y5-2b和C17的增殖代谢表现更优,在20 h时活菌数达到稳定,增长至9.0 lg(CFU/mL);发酵梨汁的抗氧化活性显著升高(P<0.05);糖含量降低,其中葡萄糖消耗最多,利用率为5.29%~8.85%;部分有机酸含量呈现下降,在Y5-2b和C17组未检出苹果酸,CH-2组苹果酸含量与未发酵的含量无显著差异(118mg/100mL),且发酵后柠檬酸含量分别下降了 33.65%,42.97%,38.71%;不同乳酸菌发酵对梨汁的酚类物质含量具有不同的影响,以熊果苷、儿茶素、芦丁含量变化为主;从梨汁样品中共鉴定出44种香气成分,其中3-羟基-2-丁酮、正丁醛、丁酸等具有果香的挥发性风味物质含量在发酵后均有不同程度的增多.结论:乳酸菌发酵赋予梨汁丰富的营养物质、风味和较高的抗氧化活性,为乳酸菌发酵梨汁提供了一定的理论依据.

Abstract

In order to improve the antioxidant activity and flavor of snow pear juice,pear juice was fermented with Lac-tobacillus casei Y5-2b,Lactobacillus plantarum C17 and Lactobacillus acidophilus CH-2,respectively.Physicochemical properties and antioxidant activities were characterized.And high-performance liquid chromatography and gas chromatogra-phy-ion mobility spectrometry(GC-IMS)was applied to analyze the nutrients and aroma components.Results showed that the three strains had good growth and metabolism in pear juice.Moreover,the proliferation metabolism of Y5-2b and C17 showed better performance,with a stable number of viable bacteria reaching 9.0 lg CFU/mL at 20 hours.The antioxidant activity of fermented pear juice increased significantly(P<0.05).The sugar content decreased,with glucose being the most consumed and the utilization rate ranging from 5.29%to 8.85%.Partial organic acid content showed a decrease,with no malic acid detected in groups Y5-2b and C17.There was no significant difference in malic acid con-tent between the CH-2 group and the unfermented group(1 18 mg/100 mL),and the citric acid content decreased by 33.65%,42.97%,and 38.71%respectively after fermentation.Different lactic acid bacteria fermentation had different ef-fects on the content of phenols in pear juice,mainly arbutin,catechin and rutin.A total of 44 aroma components were identified from juice samples,of which 3-hydroxy-2-butanone,n-butyraldehyde,butyric acid and other characteristic flavor substances with fruit aroma increased in varying degrees after fermentation.In conclusion,lactic acid bacteria en-dowed the pear juice with abundant substances,flavor and high antioxidant activity,which provides a certain theoretical basis for lactic acid bacteria fermentation of pear juice.

关键词

乳酸菌/发酵梨汁/营养成分/抗氧化/香气成分

Key words

lactic acid bacteria/fermented pear juice/nutritional composition/antioxidant/aroma component

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基金项目

河北省现代农业产业技术体系项目(HBCT2021210205)

河北省重点研发计划项目(19227114D)

河北省重点研发计划项目(21327313D)

出版年

2024
中国食品学报
中国食品科学技术学会

中国食品学报

CSTPCDCSCD北大核心EI
影响因子:1.079
ISSN:1009-7848
参考文献量17
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