首页|多菌种混合制曲生产玫瑰醋的工艺及品质研究

多菌种混合制曲生产玫瑰醋的工艺及品质研究

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为探究多菌种制曲生产对浙江玫瑰醋工艺和品质的影响,通过正交试验优化多菌种配比,将最佳两组混合菌种应用于玫瑰醋生产,并进行品质评价.结果表明,淀粉酶和蛋白酶酶活力最高的菌种配比分别为红曲霉∶米曲霉∶黑曲霉∶根霉为1∶1∶1∶1和1∶1∶1∶2,按此比例进行制曲混合,并用于酿造的发花工艺.整个发酵工艺跟踪分析显示,玫瑰醋的多边发酵过程能顺利完成,两组发酵成品的总酸度分别为6.97 g/100 mL和6.63 g/100 mL,得率比传统方法提高17.21%和11.29%.在两组玫瑰醋试验样品中检测到醋酸、乳酸、苹果酸、柠檬酸等8种呈味有机酸,非挥发性酸含量分别为56.04 mg/L和57.85 mg/L不逊色于传统玫瑰醋41.053 mg/L.采用顶空固相微萃取-气相-质谱联用方法共鉴定出44种挥发性风味物质,两组的挥发性风味物质含量分别6 348.14 mg/L和6 791.75 mg/L,略高于传统的5 030.07 mg/L.感官评价表明,多菌种制曲生产的玫瑰醋在甜味、酯香气味上略有提高,特别是 1∶1∶1∶2多菌种制曲组感官评分值最高.本研究为传统玫瑰醋生产的制曲机械化改进提供了参考.
Research on the Process and Quality of Rosy Vinegar Production by Multi-strain Mixed Cranking
To investigate the impact of multi-strain koji production on the process and quality of Zhejiang Rose Vinegar,a study was conducted to optimize the multi-strain ratio using an orthogonal experiment.The two best-performing mixed strains were applied to the production of rose vinegar,and a quality evaluation was performed.The results indicated that two ratios with the highest amylase and protease activities were 1∶1∶1∶1 and 1∶1∶1∶2 of Monascus purpureus∶Aspergillus oryzae∶A.niger∶Rhizopus,which were prepared and subsequently applied to the vinegar brewing process.Fermentation tracking showed that the multi-strain fermentation process for rose vinegar was successfully completed,with the total acidity of the final products reaching 6.97 g/100 mL and 6.63 g/100 mL,respectively,representing a yield increase of 17.21%and 11.29%compared to the traditional method.Eight flavor-contributing organic acids,including acetic acid,lactic acid,malic acid,and citric acid,were detected in the two rose vinegar samples,with non-volatile acid contents of 56.04 mg/L and 57.85 mg/L,respectively,which were comparable to the 41.053 mg/L of traditional rose vinegar.A to-tal of 44 volatile flavor compounds were identified using headspace solid-phase microextraction combined with gas chro-matography-mass spectrometry(HS-SPME-GC-MS),with volatile flavor compound contents of 6 348.14 mg/L and 6 791.75 mg/L in the two groups,slightly higher than the 5 030.07 mg/L in traditional vinegar.Sensory evaluation revealed that rose vinegar brewed with multi-strain koji showed slight improvements in sweetness and ester aroma,with the 1∶1∶1∶2 multi-strain group achieving the highest sensory scores.This study provides a reference for the mechanized improve-ment of koji production in traditional rose vinegar manufacturing.

multi-strain fermentationrosy vinegarorganic acidvolatile flavor substances

张林祥、周利南、邢利民、冯纬、贺德贵、蒋予箭、朱军莉

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浙江工商大学食品与生物工程学院 杭州 310018

杭州市食品酿造有限公司 浙江湖州 313200

湖州老恒和酿造有限公司 浙江湖州 313000

多菌种发酵 玫瑰醋 有机酸 挥发性风味物质

浙江省科技厅公益项目

LGG19C200001

2024

中国食品学报
中国食品科学技术学会

中国食品学报

CSTPCD北大核心EI
影响因子:1.079
ISSN:1009-7848
年,卷(期):2024.24(9)
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