摘要
为研究电场处理对储藏大豆原油氧化稳定性的影响.以电场强度和处理时间为单因素,分析其对油脂过氧化值(POV)的影响,确定最佳电场处理条件.采用GC-MS法和HPLC法测定电场处理对油脂甾醇、角鲨烯和生育酚含量的影响,并评价电场处理后油脂体外抗氧化能力.结果表明,电场处理的最佳电场强度为3 000V/m,电场处理时间为3h.电场处理对油脂内源性抗氧化成分甾醇、角鲨烯和生育酚的保护效果显著,经电场处理的油脂甾醇相对损失抑制率达23.08％,角鲨烯相对损失抑制率达19.99％,对生育酚个体具有不同的损失抑制程度,保护程度由强至弱为δ-生育酚＞β-生育酚＞α-生育酚＞γ-生育酚,其中电场对大豆原油中δ-生育酚的损失抑制率影响明显.电场处理减缓了油脂DPPH、ABTS+自由基清除活性能力和FRAP铁离子还原能力的下降速率,保护了油脂的抗氧化能力.电场可抑制油脂的氧化速度,减缓氧化程度,并对油脂内在抗氧化成分损失有抑制作用,在一定程度上保护了油脂的抗氧化能力,具有较好的抗氧化效果.
Abstract
To study the effect of electric field treatment on oxidation stability of soybean crude oil.Based on the influ-ence of single factor about electric field intensity or treatment time on lipid peroxide value(POV),the optimal condi-tions of electric field treatment were determined.The content changes of sterol,squalene and tocopherol under the opti-mal electric field condition were determined by GC-MS and HPLC.The antioxidant capacity of oils with the electric field treatment was evaluated in vitro.The optimal electric field intensity was 3 000 V/m and the electric field treatment time was 3 h.The relative loss inhibition rate of sterol,squalene and tocopherol were 23.08％and 19.99％,respectively.The loss inhibition rate of sterol and squalene were different for tocopherol.The protection order was δ-tocopherol＞β-toco-pherol＞α-tocopherol＞γ-tocopherol,and the electric field had a relatively obvious effect on the inhibition rate of δ-to-copherol loss in soybean crude oil.Electric field treatment slowed down the decrease rate of DPPH and ABTS+free radi-cal scavenging activity and FRAP reducing ability of oils,and protected the antioxidant capacity of oils.Electric field can inhibit the oxidation rate and the degree of oxidation of oils,and has an inhibitory effect on the loss of antioxidant components in oils,which protects the antioxidant capacity of oils and has a good antioxidant effect.
维普
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