中国食品学报2024,Vol.24Issue(11) :95-106.DOI:10.16429/j.1009-7848.2024.11.010

乳清蛋白-海藻酸二醛乳液的消化特性研究

Studies on Digestive Characteristics of Whey Protein-alginate Dialdehyde Emulsions

吴莎萍 吴长玲 吴峰华 何志平 刘兴泉 王鹏
中国食品学报2024,Vol.24Issue(11) :95-106.DOI:10.16429/j.1009-7848.2024.11.010

乳清蛋白-海藻酸二醛乳液的消化特性研究

Studies on Digestive Characteristics of Whey Protein-alginate Dialdehyde Emulsions

吴莎萍 1吴长玲 1吴峰华 1何志平 1刘兴泉 1王鹏1
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作者信息

  • 1. 浙江农林大学食品与健康学院 杭州 311300
  • 折叠

摘要

为研究乳清蛋白(WPI)和海藻酸二醛(ADA)间的相互作用对O/W乳液中姜黄素体外消化的影响,采用紫外光谱、荧光光谱和红外光谱分析探究WPI和ADA间的相互作用,通过测定液滴尺寸、流变性、界面吸附率、乳化活性、浊度和分析姜黄素的释放特性研究复合物对乳液的影响.结果表明,在动态可逆共价键-席夫碱键的驱动下,WPI-ADA复合物改善了乳液的界面吸附率、乳化活性、液滴尺寸和浊度.与WPI乳液相比,WPI-ADA复合物乳液的界面吸附率和乳化活性等均有所提高.当ADA添加量为WPI的0.8%时,乳液的液滴尺寸较小(214.5 nm)、浊度较低(30 061.8),乳化活性指数(295.2 m2/g)和乳化稳定性(0.617)较高.此外,WPI-ADA复合物乳液在模拟胃肠液中具有较高的稳定性,并且提高了姜黄素的生物利用度.

Abstract

The interactions between whey protein(WPI)and alginate dialdehyde(ADA)identified by UV-vis spec-troscopy,fluorescence spectroscopy and infrared spectroscopy were investigated for effects on the in vitro digestive proper-ties of curcumin in O/W emulsions.The influence of the complexes on the emulsions was studied by droplet size,rheo-logical properties,interfacial adsorption rate,emulsifying activity,turbidity and curcumin release characteristics.The re-sults showed that the interfacial adsorption rate,emulsifying activity,droplet size and turbidity of emulsions driven by dynamic reversible covalent bond-Schiff base bond were improved.Compared with WPI emulsions,WPI-ADA complexes emulsions had higher interfacial adsorption rate and emulsifying activity.When the amount of ADA added was 0.8%of WPI,the emulsions had smaller droplet size(214.5 nm),lower turbidity(30 061.8),higher emulsifying activity(295.2 m2/g),and better emulsifying stability(0.617).Furthermore,the WPI-ADA complexes emulsions,which were more sta-ble in simulate gastrointestinal fluid,promoted the bioavailability of curcumin.

关键词

疏水性活性物质/蛋白-多糖复合物/分子间相互作用/体外消化

Key words

hydrophobic active substance/protein-polysaccharide complex/intermolecular interaction/in vitro digestive properties

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出版年

2024
中国食品学报
中国食品科学技术学会

中国食品学报

CSTPCDCSCD北大核心EI
影响因子:1.079
ISSN:1009-7848
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