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浓香型白酒发酵过程中黄水细菌群落结构的演替规律

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在浓香型白酒发酵过程中不同层次的酒醅发酵过程存在差异,如底层酒醅会受黄水浸泡发酵的影响,而黄水中细菌群落结构对有机酸合成有很大影响.为明确黄水中细菌群落结构的演替规律及有机酸代谢情况,通过高通量测序技术解析黄水细菌群落的动态变化,分析发酵过程细菌群落结构与有机酸含量的相关性.采用PICRUSt分析对黄水中细菌群落的功能进行了预测.结果表明,依据发酵过程中黄水的细菌群落结构变化可将发酵过程分为2个阶段:发酵前期(5~20 d)与发酵后期(20~60d),这2个阶段的的细菌群落结构差异显著(P<0.05).发酵过程中乳酸杆菌属是黄水的绝对优势菌属,发酵5~10d相对丰度在97%以上,产己酸菌属、梭菌属、氢孢菌属、岩石单胞菌属、嗜蛋白菌属等16个细菌属的相对丰度在发酵后期明显增加.PICRUSt分析表明,黄水细菌群落在发酵过程中可能主要参与糖酵解途径和酸代谢途径,与乳酸、乙酸、己酸和丁酸相关的功能酶相对丰度较高,说明黄水中细菌群落具有产香的能力,对浓香型白酒酒醅发酵具有重要影响.
Succession of Bacterial Community Structure in Huangshui during Fermentation Process of Strong Aromatic Chinese Spirit
In the fermentation process of strong aromatic Chinese spirit,the different levels of fermented grains was un-even,the bottom fermented grains was affected by Haungshui immersion fermentation,and the bacterial community struc-ture from Huangshui had a great contribution to organic acid metabolism.To identify the succession and potential func-tion of bacterial community structure in Huangshui during the fermentation process,high-throughput sequencing was bor-rowed to reveal the succession of bacterial community structure,the relationship between bacterial community structure and the content of organic acid content was analyzed in different fermentation stages.PICRUSt analyse was used to pre-dict the potential function of bacterial community in Huangshui.The results showed that the changes of bacterial commu-nity structure in Huangshui could be divided into two stages during the fermentation process,5-20 d and 20-60 d,re-spectively.Lactobacillus was the absolute dominant bacterial genus in Huangshui,with a relative abundance of more than 97%at 5-10 d.The relative abundance of Caproiciproducensz,Clostridium,Petrimonas,Hydrogenispora,Proteiniphilum increased significantly in late fermentation stages.PICRUSt analysis showed that Huangshui bacterial communities may mainly participate in glycolytic pathways during fermentation.The relative abundance of functional enzymes related to lac-tic acid,acetic acid,caproic acid,and butyric acid was relatively high.The results showed that the bacterial community in Huangshui has great potential for aroma-producing and an important impact on the fermentation of strong aromatic Chinese spirit fermented grains.

strong aromatic Chinese spiritHuangshuibacterial community structureorganic acid

曾波、郑佳、冯政、黄治国、苏建、邓杰

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四川轻化工大学 酿酒生物技术及应用四川省重点实验室 四川宜宾 644000

宜宾五粮液股份有限公司 四川宜宾 644000

中国轻工业酿酒生物技术及智能制造重点实验室 四川宜宾 644000

浓香型白酒 黄水 细菌群落结构 有机酸

2024

中国食品学报
中国食品科学技术学会

中国食品学报

CSTPCD北大核心EI
影响因子:1.079
ISSN:1009-7848
年,卷(期):2024.24(11)