Objective:To analyze the differences of goji flavonoids from different origins from the perspective of pro-teomics.Methods:The protein differences of goji berries from different origins were explored by tandem mass tag(TMT)combined with liquid chromatography-tandem mass spectrometry(LC-MS/MS)technology in Ningxia,Qinghai and Gansu.Results:A total of 716 differential expressed proteins(DEPs),and a total of four metabolic pathways associated with flavonoids were found in the KEGG pathway,and the phenylpropane biosynthesis pathway was significantly enriched in all three comparison groups(P＜0.05),indicating that the phenylpropane biosynthesis pathway might make a greater contribution to the synthesis of goji flavonoids.Further screening of 24 key differentially expressed proteins related to flavonoid synthesis,it was found that phenylalanine ammonia-lyase(PAL),cinnamic acid 4-hydroxylase(C4H),chal-cone isomerase(CHI),chalcone synthase(CHS),etc.might be important DEPs for the biosynthesis of flavonoids in Ningxia,Qinghai and Gansu.Conclusion:The differential proteins related to the biosynthesis of goji flavonoids were i-dentified from the protein level,and the anabolic pathway of goji flavonoids was preliminarily speculated,which laid a foundation for exploring the differences in the biosynthesis of goji flavonoids in different origins.
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