Recognition of Amines by Pyran Derivatives and Evaluation of Fish Meat Freshness Using Tags
The traditional TVB-N method for evaluation of freshness has defects such as complicate process,delay result and other defects.Therefore,it is extremely significant to develop a rapid,non-destructive and real-time approach for detecting the freshness of salmon.In this paper,a benzopyran derivative HDX was constructed through condensation re-action.HDX could sensitively recognize 10 amines,moreover,within the EtOH/H2O(volume ratio 1∶9)system,obvious color and fluorescence changes occurred within 5 s.The detection limit of HDX for spermine was 0.75 μmol/L.By utiliz-ing HDX,fluorescence imaging of spermine and putrescine could be achieved in living cells.The sensing label HDXIL was prepared by loading HDX onto filter paper,and HDXIL could respond for fish freshness by colorimetric and fluores-cence dual channels.This sensor label HDXIL showed significant recognition effect on different grades of salmon meat(distinguished by three colors).Its evaluation results were highly accurate and trustworthy,and it was suitable for real-time and on-site monitoring of the freshness of salmon meat.