Feeder layer cells have been used in the in vitro expansion of NK cells,TIL cells,etc.,since they can promote the expansion efficiency of target cells.Trophoblast cells originate from diverse sources and undergo various modifications.Taking genetically edited K562 cells as an example,this paper summarizes the characteristics and latest research progress of feeder layer cells,focusing on their function,cell origin,construction of engineered cell lines and cell bank,production process,quality research and quality control,and strategies for their use and control.The CMC evaluation considerations and discussions are presented to provide references and insights for the industry and regulatory agencies,expecting to promote the clinical transformation and application of such products.
关键词
滋养细胞/基因编辑的K562细胞/药学评价/使用和控制
Key words
feeder layer cells/genetically modified K562 cells/CMC assessment/use and control
维普
万方数据