Objective:This study aims to determine the contents of saikosaponins c,a,and d in Xiaoyao granules using the RP-HPLC-ELSD method.Methods:An agilent SB-C18,chromatographic column(4.6mm×250mm,5µm)was utilized.The mobile phase consisted of acetonitrile(A)and water(B).The gradient elution procedure was as follows:0～18min,30%A;18～26min,35%～45%A;26～35min,45%～55%A.The flow rate was 1.0ml/min,the column temperature was 30℃,the gas flow rate was 2.5L/min,the evaporation temperature was 60℃,and the injection volume was 10µl.Results:Saikosaponins c,a,and d exhibited good linear relationships within the ranges of 0.9500～19.0000µg,0.1088～21.7600µg,and 1.5200～30.4000µg,respectively.The average recovery of saikosaponin c was 99.49%with an RSD of 0.35%(n=9);saikosaponin a had an average recovery of 99.99%with an RSD of 0.66%(n=9),and saikosaponin d had an average recovery of 99.12%with an RSD of 0.47%(n=9).Conclusion:The method is accurate,sensitive,and has good reproducibility.It is suitable for the determination of contents of saikosaponins c,a,and d in Xiaoyao granules.
维普
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