ITRAQ Technology and Its Application in Proteomics
In the past years, proteomics was developed quickly and its corresponding methodological research has also been made great progress, a series of new technologies blended in proteomics technology, particularly the new combination of multi-dimensional liquid chromatography and mass spectrometry ( MS) -based technologies with protein fragmentation methods, enable us easily to identify the dynamics of proteins profiles in any complex biological process. Isobaric tags for relative and absolute quantification (iTRAQ) is one of the new techniques with high sensitivity and accuracy, demonstrating a remarkable advantage in simultaneous analysis of multiple samples and subsequently providing the relative quantification on hundreds of proteins at one time. The iTRAQ reagent produced high quality, reproducible result in enriched complexes, organelles, and whole cell lysates. Hence iTRAQ has been widely used in areas of life science. This review will focus on the principle, experimental procedure and application of iTRAQ in the last few years.
isobaric tags for relative and absolute quantification (iTRAQ)proteomicstandem mass spectrometrymulti-dimensional liquid chromatographybiomarker
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