Histone H3 Lysine 27 Methylase and Demethylase Gene Expression was Regulated by the MEK-ERK Pathway
Many studies have shown that overexpression of H3K27 methylase gene EZH2 may be regulated by the MEK-ERK signaling pathway in some cancer cells. In order to determine whether it also exists in mice and the MEK-ERK signaling pathway could also involve the H3K27 methyltransferase gene EZH1 and H3K27 demethylase genes UTX and JMJD3, we used RT-PCR and Western blot methods to detect the expression of EZH1, EZH2, UTX and JMJD3 genes with the different concentrations of the MEK-ERK inhibitor U0126 (0, 10, 20, 40 (xmol/L) in three murine cell lines C2C12, C127 and NIH3T3. The results showed that the expression of H3K27 mehylase genes EZH1 and EZH2 decreased in the three cell lines compared with the control and the change of EZH2 gene expression reached the significant level (P ＜ 0. 05) in C2C12 and NIH3T3 two cell lines. The expression of UTX and JMJD3 genes increased and that of JMJD3 gene reached the significant level ( P ＜ 0. 05 ) . Therefore, in the murine cell lines the MEK-ERK signaling pathway may be involved in the regulation of EZH2 and JMJD3 genes, but the role on EZH1 and UTX genes is not obvious.
MEK-ERK pathwayEZH1 /EZH2UTX/JMJD3H3 K27 me3C2 C12/C127/NIH3 T3
NETL
NSTL
万方数据
维普
