Hydrogen Sulfide Mediates Sulfhydration and Its Chemical Detection Techniques
Hydrogen sulfide(H2S)is the third endogenous gasotransmitter,following nitric oxide and carbon monoxide,which regulates various physiological and pathological processes by influencing cellular signaling pathways.In recent years,research has found that the effects of H2S are mainly mediated through sulfhydration of target proteins,which can alter their structure,affect their activity,stability and protein-protein interactions,thus regulating cellular signaling pathways and related biological processes.This article reviews the current research of sulfhydration and provides a detailed summary of the known sulfhydration modifications and their specific cysteine(Cys)sites that have been identified.This article further discusses the relationship between sulfhydration and nitrosylation.It also covers the types of reac-tions involved in sulfhydration modification and describes the principles and characteristics of three chem-ical detection methods:the maleimide assay,the modified biotin switch assay,and the tag-switch assay.By comparing the conditions and applications of sulfhydration detection using these three methods,this article further summarizes the important procedures during the detection process.Based on our experience in sulfhydration research,this article focuses mainly on the cell and tissue sample preparation with de-tailed experimental procedures for the maleimide assay.Additionally,it discusses the limitations of cur-rent sulfhydration detection methods and the unresolved issues in this field,which may provide references for further research in sulfhydration.
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NSTL
万方数据
维普
