Transcriptome Analysis of Mytilus coruscus Mantle Revealed the Effects of Urea on Mussel's Tolerance to Seawater Acidification
Ocean acidification,as one of global environmental problems,is considered detrimental to ma-rine calcifiers and poses a serious threat to the survival of marine mineralized species.Shellfishes are the main species in marine aquaculture,and ocean acidification will inhibit the biomineralization process for the calcified shells,and thusly have serious impacts on the development of shellfish aquaculture.Previ-ous studies have found that urea plays an auxiliary role in mussel shell formation,and it is speculated that urea helps mussels survive during ocean acidification.To further explore the molecular mechanism through which urea has a positive effect on mussel's tolerance to ocean acidification,transcriptomic strat-egies were used to analyze the changes in gene expression levels of mussel's mantle tissues induced by u-rea supplementation in acidified seawater.The results showed that urea supplementation reversed the transcriptome changes of mussel mantle under acidification,and inhibited autophagy,apoptosis and im-mune stress-related pathways activated by acidification stress.In addition,urea induced the up-regulation of the expression of some shell matrix proteins,which helped to maintain the biomineralization process of mussels under acidification conditions.Our research provided clues for understanding the mechanism of mussel tolerance to ocean acidification,as well as provided new ideas for the healthy development of shellfish aquaculture in ocean acidification.
NETL
NSTL
万方数据
维普
