Artemisinin Regulates 4T1 Breast Cancer Cell Migration and Apoptosis Through the TIGIT/CD155 Signaling Axis
Breast cancer is one of the malignant tumors that seriously affects women's lives and health.In recent years,the anti-tumor effect of artemisinin(ART),an active ingredient of traditional Chinese medicine,has become more prominent.However,the effectiveness of ART in the treatment of breast cancer metastasis and its mechanism are still unclear.In this paper,we used the mouse breast cancer cell 4T1 and mouse mammary epithelial cell HC11 to explore the role of ART in inhibiting metastasis and inducing apoptosis.The MTS assay showed that ART was able to significantly inhibit the viability of 4T1 cells in a concentration and time-dependent manner.Analysis of cell colony formation,Transwell and cell scratch results revealed that ART significantly inhibited the metastasis of breast cancer 4T1 cells.Flow cytometry results showed that ART promoted ROS production,loss of mitochondrial membrane potential,cell cycle arrest and thus apoptosis in tumor cells.Bioinformatic analysis methods and studies confirmed that extremely significant high expression of TIGIT(P＜0.001)was closely associated with breast cancer metastasis,and that ART was able to significantly reduce the gene expression level of the TIGIT/CD155 signaling axis(P＜0.001,P＜0.01).In summary,this study reveals the regulatory mechanism by which ART may inhibit breast cancer metastasis by blocking the TIGIT/CD155 signaling axis,which pro-vides a basis for ART as a potential therapeutic agent for breast cancer,and contributes to the exploration of a new way of treating tumors using the active ingredients of Chinese herbal medicine.
artemisininbreast cancer metastasisapoptosisTIGIT-CD155 signal axis
NETL
NSTL
万方数据
维普
