Since March this year,the World Health Organization no longer classified severe acute respir-atory syndrome coronavirus 2(SARS-CoV-2)as a catastrophic health event of global concern.But the number of new infections is still increasing,which is due to the failure of specific antibodies and immune escape caused by frequent mutations in the virus gene.At present,specific therapeutic drugs are still limited and lack broad spectrum.The viral S protein is the key to viral infection in human cells.Herein we show that alkaline serine protease from Neanthes japonica,pI 4.4(ASPNJ)could efficiently degrade the S protein of omicron variant strains BA.2,BF.7 and XBB.1,whereas the mutation S proteins ob-tained stronger resistance to ASPNJ digestion.Preliminary experiments showed that ASPNJ may reduce the pseudovirus infection into 293T-ACE2.ASPNJ can widely and efficiently degrade the S protein.Thus,our results suggested that ASPNJ may become a new tool enzyme for studying the structure and function of SARS-CoV-2.
关键词
新冠病毒/刺突蛋白/沙蚕碱性丝氨酸蛋白酶/变异新冠病毒/假病毒
Key words
SARS-CoV-2/spike protein(S)/alkaline serine protease from Neanthes japonica(ASPNJ)/SARS-CoV-2 mutation/pseudovirus
维普
万方数据