miR-485-5p Inhibits the Proliferation,Migration and Invasion of Prostate Cancer Cells by Targeting OGT
Prostate cancer is one of the most common malignant tumors of the male reproductive system.Although strategies such as androgen deprivation therapy are effective in treating prostate cancer,most patients will eventually progress to castration-resistant prostate cancer for which there is no clinically ef-fective treatment.Therefore,it is particularly critical to find new and more effective therapeutic targets.Many studies have shown that the abnormal expression of microRNAs(miRNAs)is closely related to the occurrence of tumors.It has been reported that miRNA-485-5p(miR-485-5p)functions as a cancer sup-pressor in most tumors,but its role in prostate cancer remains largely unknown.This study aimed to ex-plore the role and mechanism of miR-485-5p in the proliferation,migration and invasion of prostate canc-er.Bioinformatics prediction and qRT-PCR detection showed that the expression of miR-485-5p was down-regulated in prostate cancer.Transfection of miR-485-5p mimics could inhibit the proliferation,mi-gration and invasion of prostate cancer cells confirmed by plate cloning,Transwell and scratch healing ex-periments.Bioinformatics prediction,Western blotting and qRT-PCR detection confirmed that O-linked N-acetylglucosamine transferase(OGT)is a direct target of miR-485-5p.Further examination showed that OGT was highly expressed in prostate cancer,and transfection with OGT siRNA could significantly inhibit the proliferation,migration and invasion of prostate cancer cells and could effectively reverse the effect of miR-485-5p inhibitor.In summary,miR-485-5p can inhibit the proliferation,migration and in-vasion of prostate cancer cells by negatively regulating OGT.The results of this study are helpful to fur-ther elucidate the role and mechanism of miR-485-5p in the development and development of prostate cancer,and can provide experimental basis for searching for therapeutic targets of prostate cancer.
万方数据
维普
