Nucleic Acid-barcoding Technologies:A New Method for Extending High-throughput Protein-Protein Interactions
Protein-protein interaction(PPI)participates in almost all important biological processes in the organism and plays a vital role in the basic life process of cells.It is of great biological significance to develop a high-throughput PPI detection method.At present,the next-generation sequencing(NGS)technology has developed rapidly and can determine more than 1 billion template DNA sequences in a few days.Due to the unique sensitivity,specificity,high throughput,and multiplexing advantages of parallel DNA sequencing technology,it has been used as a broad-spectrum molecular counter and applied to genome sequencing,transcriptome sequencing and other fields.The nucleic acid barcode technology realizes the high-throughput detection of PPI by connecting the oligonucleotide tag with the target proteins to mark the encoded protein,and then using the high-throughput sequencing method to detect the interacting protein.This technology promotes the rapid development of PPI detection methods,improves the throughput of single experiment detection,and provides a strong technical support for building PPI network.In this paper,we describe in detail the design,generation and reading of nucleic acid barcodes in the PPI detection method.By analyzing the application examples of the nucleic acid barcode technology in PPI research,this paper discusses their advantages and disadvantages,evaluates the reliability of data,and discusses the future development trend of PPI detection methods based on the nucleic acid barcode technology.
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