Enzyme-mediated Proximity Cell Labeling Methods to Probe Cell-Cell Interactions
Cell-cell interactions(CCIs)can occur through the formation of intercellular synapses mediated by cell surface proteins,glycans,lipids,to maintain body homeostasis and regulate physiological functions.These CCIs are complex,involving the participation of many different cell surface and intracellular molecules.Therefore it is key to accurately identify,characterize and quantify cell-cell interactions.In recent years,the technical means of researching CCIs have been continuously introduced,among which proximity labeling is a promising chemical biology method for studying cell-cell interactions.Currently,there are mainly two types of labeling strategies.One is to rely on the direct binding between enzymes expressed on the surface of"bait"cells by genetic engineering and receptor substrates on adjacent cells to achieve intercellular proximity labeling.The other is to use enzymes or organocatalysts(such as photocatalysts)that are recombinantly expressed by genetic engineering or coupled to the surface of"bait"cells by chemical(chemoenzymatic)methods,and following appropriate stimulation or activation,targeted delivery of labeling molecules are carried out.Between them,the enzyme-mediated proximity cell labeling methods have promising application value in the detection and characterization of CCIs.This review defines methods involving enzymes during the labeling process as enzyme-mediated proximity cell labeling methods.A remarkable advantage of this approach is the small labeling radius that can be achieved due to direct physical contacts between the enzymes and receptor substrates or enzyme-catalyzed generation of highly reactive labeling molecules.We summarize the principles,advantages and disadvantages,and existing applications of the enzyme-mediated proximity cell labeling methods developed in recent years.
NETL
NSTL
万方数据
维普
