首页|细胞分裂周期蛋白73基因缺失抑制粟酒裂殖酵母细胞的有性生殖和有丝分裂

细胞分裂周期蛋白73基因缺失抑制粟酒裂殖酵母细胞的有性生殖和有丝分裂

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cdc73基因编码粟酒裂殖酵母(Schizosaccharomyces Pombe)RNA聚合酶Ⅱ辅助因子Cdc73，参与G2期检查点激活并调控细胞周期。但cdc73基因缺失对细胞有丝分裂动力学的调控尚不清楚。本研究采用活细胞成像、荧光蛋白标记技术探究粟酒裂殖酵母cdc73基因缺失后对细胞有性生殖和细胞有丝分裂中微管、肌动蛋白、线粒体和组蛋白动力学的影响。结果表明:在有性生殖中，cdc73基因缺失会导致子囊孢子长度增加14。23％，产4个孢子数量的细胞减少64。08％;活细胞成像结果分析发现，在有丝分裂中，分裂后期微管伸长的长度缩短11。21％，伸长时间减少17。39％;肌动蛋白环形成和收缩速率分别降低33。33％和26。09％，形成和收缩时间分别延长58。00％和40。38％;同时，肌动蛋白环、线粒体和组蛋白表达量都增加。本研究揭示了cdc73基因缺失可抑制有丝分裂中纺锤体的伸长，延缓肌动蛋白环的形成与收缩，为进一步探寻Cdc73蛋白在细胞分裂中参与调控微管和肌动蛋白动力学功能提供了一定的科学依据。
The Cell Division Cycle 73(Cdc73)Deletion Mutant Inhibits Sexual Reproduction and Mitosis of Fission Yeast Cells
The cdc73(cell division cycle 73)gene encodes the RNA polymerase Ⅱ cofactor Cdc73 in fis-sion yeast(Schizosaccharomyces Pombe),and is involved in G2 checkpoint activation and regulates the cell cycle.However,whether Cdc73 regulates cell mitotic dynamics is unknown.In this study,fluores-cent protein labeling and live cell imaging techniques were used to investigate the effects of cdc73 deletion on sexual reproduction and the dynamics of microtubules,actin,mitochondria,and histones during mito-sis.The results showed that in sexual reproduction,cdc73 deletion resulted in a 14.23％increase in the length of ascospores and a 64.08％decrease in the number of cells producing four spores.Analysis of the live cell imaging results revealed that,in mitosis,the elongation length of microtubules in anaphase was shortened by 11.21％,and the elongation time was reduced by 17.39％;the formation and contraction rates of actin rings decreased by 33.33％and 26.09％,respectively,and the formation and contraction times were prolonged by 58.00％and 40.38％,respectively.Meanwhile,the expression levels of actin ring,mitochondrion,and histones also increased.This study revealed the cdc73 deletion inhibits spindle elongation and delays actin ring formation and contraction in mitosis,which provides some scientific basis for further exploring the involvement of Cdc73 in regulating microtubule and actin dynamics in cell divi-sion.

cell division cycle 73(cdc73)geneSchizosaccharomyces pombesexual reproductionmi-tosiscell dynamics

刘梦楠、白鑫、余雯、李欣霖、丁祥、侯怡铃

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西华师范大学生命科学学院西南野生动植物资源保护教育部重点实验室,四川南充 637009

西华师范大学环境科学与工程学院嘉陵江流域生态环境保护与污染防治南充市重点实验室,四川南充 637009

cdc73基因 粟酒裂殖酵母 有性生殖 有丝分裂 细胞动力学

四川省科技厅应用基础面上项目四川省科技厅农业科技成果转化资金项目四川省转移支付科技成果转移转化示范项目四川省转移支付科技成果转移转化示范项目

2022NSFSC01072022NZZJ000322ZYZFSF000923ZHSF0082

2024

10.13865/j.cnki.cjbmb.2024.04.1481

中国生物化学与分子生物学报
中国生物化学与分子生物学会 北京大学

中国生物化学与分子生物学报

CSTPCD北大核心
影响因子：0.617
ISSN：1007-7626
年,卷(期)：2024.40(6)
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