首页|长非编码RNA PART1抑制喉鳞癌细胞增殖和侵袭

长非编码RNA PART1抑制喉鳞癌细胞增殖和侵袭

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长非编码 RNA(long non-coding RNA，lnc RNA)PART1 可作为竞争性内源 RNA(competing endogenous RNAs，ceRNA)，在多种肿瘤的发生发展中发挥关键作用，然而PART1在喉鳞癌(laryn-geal squamous cell carcinoma，LSCC)中的研究尚属空白。我们基于前期lncRNA测序数据发现，PART1在LSCC组织中显著低表达。进一步分析TCGA等公共数据库中的测序和临床数据，共发现了 LSCC癌/癌旁组织中146个差异表达的lncRNA(95个上调，51个下调)和2 424个差异表达的mRNA，结果显示，PART1在LSCC中普遍低表达(P＜0。0001)，且PART1高表达组预后明显更好(P＜0。05)。本文采用生物信息学手段构建了 LSCC中PART1的ceRNA调控网络，并确定与之相互作用的miRNA与mRNA。在体外验证了 PART1对LSCC细胞的重要性。通过过表达载体，显著提高了 PART1在LSCC细胞中的表达量(P＜0。001);5-乙炔基-20-脱氧尿苷染色实验、凋亡分析、划痕愈合实验、Transwell实验和鬼笔环肽染色等研究结果显示，体外过表达PART1可以显著影响LSCC细胞的增殖、凋亡、迁移和侵袭(P＜0。001)。因此，PART1可能参与并抑制LSCC的发生和发展。本研究将为阐明PART1在LSCC中的作用提供理论依据。
Long non-coding RNA PART1 Inhibits Proliferation and Invasion of Laryngeal Squamous Carcinoma Cells
Long non-coding RNA(lncRNA)PART1,a competing endogenous RNA(ceRNA),plays a crucial role in the occurrence and development of various tumors.However,research on PART1 in laryngeal squamous cell carcinoma(LSCC)remains scarce.Based on preliminary lncRNA sequencing data,we found that PART1 was sig-nificantly downregulated in LSCC tissues.Further analysis of sequencing and clinical data from public databases such as TCGA revealed 146 differentially expressed lncRNAs(95 upregulated and 51 downregulated)and 2 424 differentially expressed mRNAs when comparing LSCC tumor and adjacent tissues.The results showed that PART1 was generally downregulated in LSCC(P＜0.0001),and patients with high PART1 expression had significantly better prognosis(P＜0.05).We used bioinformatics methods to construct the ceRNA regulatory network of PART1 in LSCC and identified the miRNAs and mRNAs interacting with it.Under laboratory conditions,the im-portance of PART1 in LSCC cells was validated in vitro.Overexpression vectors significantly increased the expres-sion of PART1 in LSCC cells(P＜0.001).Experiments including 5-ethynyl-2'-deoxyuridine staining,apoptosis analysis,scratch healing assay,Transwell assay,and phalloidin staining showed that overexpression of PART1 sig-nificantly affected the proliferation,apoptosis,migration,and invasion of LSCC cells in vitro(P＜0.001).There-fore,PART1 may suppress the occurrence and development of LSCC.This study provides a theoretical basis for e-lucidating the role of PART1 in LSCC.

laryngeal squamous cell carcinoma(LSCC)PART1The Cancer Genome Atlas(TCGA)databaselong non-coding RNAs(lncRNA)bioinformatics analysis

吴昊、张文桃、贾峰峰、刘铭、朱剑军

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山西医科大学基础医学院医学细胞生物与遗传学教研室,太原 030001

太原市技术转移促进中心,太原 030006

喉鳞癌 PART1 TCGA数据库 长非编码RNA 生物信息学分析

山西省应用基础研究项目山西省应用基础研究项目山西省应用基础研究项目中国博士后科学基金

2021030212437620230212111142021030212242282023M732157

2024

10.13865/j.cnki.cjbmb.2024.05.1037

中国生物化学与分子生物学报
中国生物化学与分子生物学会 北京大学

中国生物化学与分子生物学报

CSTPCD北大核心
影响因子：0.617
ISSN：1007-7626
年,卷(期)：2024.40(7)
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