Extraction Process Optimization and Antioxidant Ability Evaluation of Inonotus obliquus Polysaccharide
[Objective] To optimize the lnonotus obliquus polysaccharide extraction by ultrasonic,and contrast the antioxidant ability of Inonotus obliquus polysaccharide from different areas.[Method] The best process of ultrasonic extraction was determined by orthogonal test method.The extraction yield and antioxidant activity of the Inonotus obliquus polysaccharide from different areas were detected.[Result] The optimal extraction conditions of ultrasonic extraction were ultrasonic time 25,m (material) /m (liquid) =1∶40,ultrasonic temperature 50℃,number of extraction 3 times.In these conditions,the extraction yield of Inonotus obliquus polysaccharide was up to 8.61g/100g.Compared with water extraction-alcohol precipitation,the extraction yield increased by more than 17.3％,and the time was shortened by 3/4.Thus,the extraction efficiency was greatly improved.The results of antioxidant activity evaluation of the polysaccharide from different areas proved that the polysaccharides obtained by ultrasonic extraction had antioxidant activity,and the antioxidant ability was sig nificant correlated with the extraction yield of polysaccharides.[Conclusion] Optimization of ultrasonic extraction significantly increased the yield of polysaccharides and kept the antioxidant activity of polysaccharides.It was a kind of feasible,practical and efficient method for the extraction of fungus polysaccharide.
国家科技期刊平台
NSTL
万方数据
维普
