Study on Extraction,Purification and Activity of Total Flavonoids from Elsholtzia bodinieri Van
[Objective]To study the purification conditions and activity of total flavonoids from Elsholtzia bodinieri Van.[Method]The single factor experiment was used to obtain the purification process of total flavonoids from Elsholtzia bodinieri Van.The β-galactosidase staining was used to observe the positive rate of aging cells.The SOD activity assay and ABTS assay were used to detect the antioxidant activity of total flavonoids from Elsholtzia bodinieri Van.[Result]The results showed that the total flavonoid content of Elsholtzia bodinieri Van was 19.375 mg/g.The AB-8 was the most suitable for the separation and purification of total flavonoids from Elsholtzia bodinieri Van.The optimal conditions for static adsorption and resolution of AB-8 were loading mass concentration 1.5 mg/mL,pH 5,adsorption for 6 h,and resolution by 70％acidified ethanol(pH 5)for 4 h,the purity increased from 46.67％to 81.62％.The optimal process conditions of dynamic adsorption and resolution were the flow rate of loading liquid was 2 mUmin,the flow rate of eluting liquid was 1 mUmin,and the elution could be achieved at 80 mL,the purity was increased to 80.33％.Antioxidant activity:compared with the control group,the posi-tive rate of senile cells was decreased by SA-β-gal(P＜0.01).The scavenging IC50 of total flavonoids on ABTS was 0.484 4 mg/mL,and the scavenging IC50 of VC on ABTS was 0.154 5 mg/mL.The SOD activity in 0.5 mg/mL and 1 mg/mL treatment groups was higher than that in injured group(P＜0.001).Antitumor activity:Total flavonoids of Elsholtzia bodinieri Van had certain inhibitory effect on the growth of HCT116 and A549,and the IC50of HCT116 was 0.848 6 mg/mL.The IC50 of A549 is 0.770 8 mg/mL,indicating that the total flavonoids of Elsholtzia bodinieri Van is a potential natural antioxidant and has certain tumor inhibition.[Conclusion]The total flavonoids obtained by optimum extraction and purification have certain antioxidant and anti-tumor activities.
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