摘要
目的:通过网络药理学探讨维生素D与肥胖的相关性,并探索影响维生素D与肥胖共同作用靶点的核心蛋白.方法:通过进入西药数据库(DrugBank数据库)检索Vitamin D的相关靶点;通过DisGeNET数据库检索Obesity的相关靶点;利用Venny平台对成分靶点和疾病靶点取交集;将共有靶点导入String构建蛋白-蛋白相互作用(PPI)网络;对药物疾病靶点做蛋白-蛋白互作网络图,并筛选蛋白与蛋白之间互相作用的核心蛋白.结果:共得Vitamin D药物靶点2个;得到肥胖疾病靶点共2 821个,取交集得到Vitamin D药物靶点及肥胖疾病靶点1个(VDR);对药物疾病靶点做蛋白-蛋白互作网络图,影响VDR作用的主要蛋白有GC、CYP27B1、MED1、EP300、RXRA、NCOA3、SMAD3、CTNNB1.结论:维生素D可能通过作用于VDR靶点发挥抗肥胖的作用.GC、CYP27B1、MED1、EP300、RXRA、NCOA3、SMAD3、CTNNB1蛋白可以与VDR相互作用,从而影响维生素D与肥胖共同作用靶点的核心蛋白.
Abstract
[Objective]To explore the interaction between vitamin D and obesity based on network pharmacology,and to explore the core proteins that affect the common targets of vitamin D and obesity.[Method]The targets of Vitamin D were obtained through the DrugBank database.The targets of Obesity were obtained through DisGeNET database.The intersection of Vitamin D targets and the targets of Obesity was obtained by the Venny platform.The common targets were imported into the String database to construct the protein-protein interaction(PPI)network,and the core proteins of protein-protein interaction were screened.[Result]Two targets of Vitamin D were obtained,and 2 821 targets of obesity disease were obtained.A common target of Vitamin D and obesity was obtained by the Venny platform,which was VDR.PPI network analysis showed that 8 proteins were the main proteins affecting the action of VDR,which were GC,CYP27B1,MED1,EP300,RXRA,NCOA3,SMAD3,CTNNB1.[Conclusion]Vitamin D may play an anti-obesity role by acting on the VDR target.GC,CYP27B1,MED1,EP300,RXRA,NCOA3,SMAD3 and CTNNB1 could interact with VDR,and affect the core protein of the common target of vitamin D and obesity.
维普
万方数据