Liquid Chromatography-Tandem Mass Spectrometry Determination of Milk and Dairy Products β-Hydroxyl Group-β-Calcium Methylbutyrate
[Objective]A liquid chromatography tandem mass spectrometer(LC-MS/MS)method was established to determinate the content of calcium β-hydroxy-β-methylbutyrate(CaHMB)in dairy products.[Method]Samples were separated by ultra-high performance liquid chromatography after extraction and purification,and then qualitatively and quantitatively determined by mass spectrometer with electrospray ion(ESI)source.[Result]The linear range of this method was 10.0—200.0 µg/L and the linear correlation coefficient was above 0.99.The detection limit of this method was 50.0 µg/kg.The rate of recovery and repeatability was 78.4%—97.9%and 1.58%—10.68%respectively when the spiked concentration was 50.0 µg/kg—5.0 g/100 g.[Conclusion]This method used liquid chromatography tandem mass spectrometer(LC-MS/MS)to efficiently and rapidly detect the content of calcium β-hydroxy-β-methylbutyrate(CaHMB)in dairy products,which could meet the detection demands with the advantages of high testing efficiency and high extraction efficiency during pre-treatment.
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