Mediating Effect Analysis of Serum Bile Acids on The Relationship Between Vegetarian Diets and Glucose Metabolism Indicators
[Objective]To investigate the differences in serum bile acids between vegetarians and omnivores,and to analyze their mediating effect on the relationship between vegetarian diets and glucose metabolism indicators.[Method]Totally 222 pairs of sex-age matched healthy adult vegetarians and omnivores recruited in the previous study were included,and the absolute concentrations of 48 bile acids and their derivatives in serum were quantitatively detected by LC-MS.Potential differential bile acids were screened using orthogonal partial least-squares discriminant analysis and Wilcoxon signed rank test.Multiple linear regressions were then employed to identify the dif-ferential bile acids and explore their relationship with fasting blood glucose(FG),fasting insulin(FI),and homeostasis model assessment of insulin resistance(HOMA-IR).Additionally,the bootstrap method was utilized to explore the mediating effect of differential bile acids on the relationship between vegetarian diets and glucose metabolism.[Result]The concentrations of 23 serum bile acids and their deriva-tives were measured,revealing significant differences between vegetarians and omnivores in glycolithocholic acid-3-sulfate(GLCA-3S),glycohyocholic acid,glycochenodeoxycholic acid(GCDCA),and glycocholic acid(GCA),all of which increased in vegetarian group.Specifically,GLCA-3S showed a negative correlation with FG,FI,and HOMA-IR,playing a mediating role in reducing FI and HOMA-IR in vegetarians;GCA and GCDCA were positively correlated with FI and HOMA-IR,masking the reduction in FI and HOMA-IR in vegetar-ians.[Conclusion]Differential bile acids included GLCA-3S,GCDCA,and GCA,may play an important role in the effects of vegetarian diets on glucose metabolism.
万方数据
维普
