Research Progress on Mycotoxin Detection Methods in Tropical Fruits and Vegetables
[Objective]The origin climate of tropical fruits and vegetables is mostly high,hot and humid,so it is easy to infect many mycotoxins,which leads to their difficult to preserve,difficult to transport,and great harm to human body.Therefore,accurate,fast and sensitive high-throughput detection methods of mycotoxin compounds are urgently needed.[Method]The new detection methods of mycotoxins in food and the characteristics of various technologies are summarized to provide reference for the detection and research of mycotoxins in tropical fruits and vegetables.[Result]At present,the analysis of the types and the content of mycotoxin in tropical fruits and vegetables is mainly divided into two parts:sample pretreatment and instrument detection.The sample pretreatment is mainly used for the extraction,purification and concentration of mycotoxins in the sample through extraction,infiltration,and chromatography.The instrumental detection methods include thin-layer analysis,HPLC,liquid chromatography-tandem mass spectrometry and enzyme-linked immunoassay.[Conclusion]Liquid chromatography-tandem mass spectrometry and fluorescence detection are the main detection methods of mycotoxin species and content.Molecular imprinting technology can eliminate the interference of small molecular substances to detec-tion signals,protect detection equipment,and reduce the amount of samples.It can be used for chromatographic mass spectrometry and chemical biological sensing,which is a new direction for accurate detection of mycotoxins.
tropical fruit and vegetablemycotoxinpre-treatment techniquedetection method
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