Characterization of Gut Microbiota and Influencing Factors of Preschool Children in Lanzhou City
[Objective]To analyze the characteristics of gut microbiota and related factors in preschool children in Lanzhou by macrogenomics technology.[Method]A total of 71 eligible healthy preschool children aged 3—7 years were enrolled,and basic informa-tion such as diet,age,gender,height and weight,as well as fecal samples were collected,and the collected feces were examined using shotgun macrogenomics technology.[Result]The gut of preschool children in Lanzhou contained a large number of beneficial bacteria,which were mainly involved in carbohydrate and amino acid metabolism. The analysis of influencing factors showed that maternal reproduc-tive age,child age and staple food groups influenced the diversity,composition and function of gut microbiota in children. As the mother's reproductive age increased,the abundance of Bifidobacterium bifidum and Romboutsia ilealis decreased in children's gut,accompanied by a decrease in metabolic functions,such as ketogenesis,chlorophyll biosynthesis,γ-glutamyl cycling,and pyrimidine ribonucleoside degradation. Clostridium leptum and groups of beneficial bacteria,such as Bacteroides dorei and Lactobacillus gasseri,were found to increase in abundance and butyric acid production in the gut of preschoolers with age,while simple carbohydrate metabolism was reduced. Potatoes and whole grains intake were associated with increased abundance of the beneficial gut microbiota Bifidobacterium bifidum,Bifidobacterium breve,and Ruminococcus bicirculans.[Conclusion]Preschool children's gut contains a large number of beneficial bacteria,and their gut microbiota is greatly influenced by their mother's reproductive age,their own age and staple foods,so focusing on the characteristics of children's gut microbiota can provide a theoretical basis and an important reference for children's health care in northwestern China.
万方数据
维普
