首页|鹅粪便中副干酪乳酸杆菌和枯草芽孢杆菌的分离及鉴定

鹅粪便中副干酪乳酸杆菌和枯草芽孢杆菌的分离及鉴定

扫码查看
目的 分离北方白鹅粪便中的副干酪乳酸杆菌和枯草芽孢杆菌,并进行鉴定.方法 收集30只52周龄北方白鹅的粪便,通过特异性培养基培养,分离获得副干酪乳酸杆菌和枯草芽孢杆菌菌株,分别编号为S00834-8和IS00439-2,并进行16S DNA测序鉴定.将获得的两2株菌株于不同培养时间(0、2、4、6、8、10、12、14、16、18、20、22、24、36和48 h)、温度(34、36、37、38、40 ℃)、pH(5.5、6.0、6.5、7.0、7.5和8.0)条件下培养,通过紫外可见分光光度计测定A600,评价菌株的生长特性.同时检测菌株的耐酸耐胆盐特性(pH为2.0、2.5、3.0、3.5、7.0,胆盐浓度为0、0.3%、0.5%、1.0%、1.5%)及对抗生素的耐受性.结果 获得的S00834-8和IS00439-2菌株分别为副干酪乳酸杆菌亚种耐受性H和枯草芽孢杆菌KA9.S00834-8菌株最佳培养条件为:于37 ℃,用pH为6.0的培养基培养12 h;具有良好的耐胆汁和耐胃酸的能力;对哌拉西林、诺氟沙星、阿米卡星、庆大霉素和四环素中度敏感,对左氟沙星、链霉素、氯霉素敏感.IS00439-2菌株最佳培养条件为:于36 ℃,用pH为7.5的培养基培养8 h;具有良好的耐胆汁和耐胃酸的能力;对左氟沙星和四环素中度敏感,对哌拉西林、诺氟沙星、链霉素和氯霉素敏感.结论 鹅源副干酪乳酸杆菌亚种耐受性H和枯草芽孢杆菌KA9具有良好的耐酸、耐胆盐特性,有望成为鹅源微生态制剂的候选菌株.
Isolation and identification of Lactobacillus paracasei and Bacillus subtilis in goose feces
Objective To isolate and identify Lactobacillus paracasei and Bacillus subtilis from feces of northern white geese.Methods Lactobacillus paracasei and Bacillus subtilis,numbered S00834-8 and IS00439-2 respectively,were isolated from feces of 30 52-week-old northern white geese and cultured in specific medium,and identified by 16S DNA sequencing.The two strains were cultured for different time duration(0,2,4,6,8,10,12,14,16,18,20,22,24,36 and 48 h),at different temperature(34,36,37,38 and 40 ℃)and pH(5.5,6.0,6.5,7.0,7.5 and 8.0).The growth characteristics of the strains were evaluated according to the A600 values measured by UV-Vis spectrophotometer.At the same time,the acid and bile salt tolerance(pH 2.0,2.5,3.0,3.5 and 7.0,bile salt concentration 0,0.3%,0.5%,1.0%and 1.5%)and the antibiotic tolerance were tested.Results The strains S00834-8 and IS00439-2 were Lactobacillus paracasei subspecies tolerance H and Bacillus subtilis KA9,respectively.The strain S00834-8 cultured under the optimum condition of the culture medium with pH 6.0,at 37 ℃,for 12 h,had good bile resistance and gastric acid resistance,which was moderately susceptible to piperacillin,norfloxacin,amikacin,gentamicin and tetracycline,and susceptible to levofloxacin,streptomycin and chlor-amphenicol.The strain IS00439-2 best cultured in the culture medium with pH 7.5,at 36℃,for 8 h,showed good bile resistance and gastric acid resistance,which was moderately sensitive to levofloxacin and tetracycline,while sensitive to piperacillin,norfloxacin,streptomycin and chloramphenicol.Conclusion Goose-derived Lactobacillus paracasei subspecies tolerance H and Bacillus subtilis KA9 have good acid resistance and bile salt resistance,which are expected to be candidate strains for goose-derived microecological preparations.

Lactobacillus paracaseiBacillus subtilisGrowth characteristicsAntibiotic resistanceGoose feces

高炳南、岳学青、王秋菊、曹雨涵、任雨龙、孙愉、魏笑、张昊

展开 >

黑龙江八一农垦大学动物科技学院,黑龙江大庆 163311

副干酪乳酸杆菌 枯草芽孢杆菌 生长特性 抗生素耐受性 鹅粪便

黑龙江省教育厅科技成果转化基地与扶贫推广资助项目中央引导地方科技发展专项中央引导地方科技发展专项

201903ZY18C07-17ZY18C07-18

2024

中国生物制品学杂志
中华预防医学会,长春生物制品研究所有限责任公司

中国生物制品学杂志

CSTPCD
影响因子:0.417
ISSN:1004-5503
年,卷(期):2024.37(2)
  • 10