Inhibitory effect of miR-628-3p on progression of ovarian cancer by targeting STK17B gene
Objective To analyze the ability of miR-628-3p to inhibit the proliferation,migration and invasion of ovarian cancer(OV)by targeting serine/threonine kinase 17B(STK17B).Methods The expression level of miR-628-3p in OV tissues and prognosis were analyzed by bioinformatics.OV cells(SKOV3,HO8910)were transfected with miR-NC(miR-NC group),miR-628-3p(miR-628-3p group)and miR-628-3p+STK17B(miR-628-3p+STK17B group).The expression level of miR-628-3p in OV cells after transfection was determined by RT-qPCR.Cells proliferation ability in each group was measured by CCK-8 and clone assays,the migration ability was detected by scratch assay,and the invasion ability was detected by Transwell invasion assay.The mass and volume of tumors were detected by tumor formation assay in nude mice.The target gene of miR-628-3p was predicted by Starbase databases,which was verified by dual luciferase reporter assay.The expression level of STK17B protein in each group was detected by Western blot.Results The expression level of miR-628-3p was low in OV tissues,and the disease-free survival(DFS)was short in patients.Compared with miR-NC group,the miR-628-3p expression was up-regulated in both SKOV3 and HO8910 cells of miR-628-3p group(t=7.789 and 7.862,respectively,each P＜0.05),the activity(t=8.124 and 8.209,respectively,each P＜0.05),numbers(t=9.012 and 9.110,respectively,each P＜0.05),migration(t=10.002 and 9.983,respectively,each P＜0.05)and invasion(t=11.245 and 11.320,respectively,each P＜0.05)abilities of OV cells were significantly decreased.Com-pared with miR-NC group,the volume and mass of transplanted tumors were significantly reduced in miR-628-3p group(t=8.429 and 10.367,respectively,each P＜0.05).It was predicted by databases that STK17B was the target gene of miR-628-3p,which was verified by dual luciferase reporter assay.The levels of STK17B mRNA(t=13.852 and 13.914,respectively,each P＜0.05)and protein(t=11.524 and 11.603,respectively,each P＜0.05)in miR-628-3p group were lower than those in miR-NC group.GEPIA data analysis showed that STK17B was highly expressed in OV tissues.Compared with miR-628-3p group,the activity(t=8.692 and 8.721,respectively,each P＜0.05),numbers(t=14.112 and 14.093,respectively,each P＜0.05),migration(t=13.805 and 13.911,respectively,each P＜0.05)and inva-sion(t=14.117 and 14.207,respectively,each P＜0.05)abilities of SKOV3 and HO8910 cells significantly increased in miR-628-3p+STK17B group.Conclusion The miR-628-3p inhibits the proliferation,migration and invasion of OV cells by down-regulating STK17B expression,thereby inhibiting the development of OV.
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