Establishment of chronic asthma mouse model induced by allergen ovalbumin and dynamic analysis on its immune response characteristics
Objective To establish a chronic asthma mouse model induced by allergen ovalbumin(OVA),and analyze the immune response dynamic characteristics.Methods Twenty-two female BALB/c mice were sensitized intraperitoneally with OVA,and the airway response was stimulated by repeated infusion through nasal cavity.Samples were taken at different time points for analysis.The bronchoalveolar lavage fluid(BALF)of mice was collected and inflammatory cells were counted;the supernatant of lavage fluid was taken and detected for the levels of various related cytokines by ELISA.The lung tissue was taken to prepare sections,which was detected for the inflammatory cell infiltration by hematoxylin-eosin(HE)staining,for the goblet cell hyperplasia in airway epithelium by periodic acid-Schiff(PAS)staining,and for the collagen deposition by Masson's Trichrome and Sircol staining.The venous blood of mice was collected,the serum was separated,and the levels of OVA-specific IgE,IgG1 and IgG2a in serum were detected by ELISA.The airway reactivity was measured using a FlexiVent small animal ventilator with low-frequency forced oscillation.Results Repeated challenges of OVA successfully induced typical asthma features such as inflammatory cell response in airway and lung tissues,inflammatory cytokine accumulation,airway goblet cell hyperplasia,lung fibrosis,allergen-specific IgE antibody response and airway hyperresponsiveness in mice,and different dynamic responsive characteristics appeared.With the repeated challenges of OVA,inflammatory cell response and goblet cell hyperplasia reduced,but collagen deposition developed,while airway hyperresponsiveness re-mained 4 weeks after the last stimulation.The expression of TGF-βl was high during the inflammatory response,while the expression of TGF-β2 and IL-33 did not increase until 4 weeks after the last challenge.Conclusion This study successfully developed a chronic asthma model in mice,showing the immune response characteristics,laying a foundation for investigating immunopathological mechanisms of chronic asthma and developing therapeutic targets and strategies.
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