Effect of gene reassortment on growth characteristics of influenza B virus
Objective To study the influence of various gene segments of influenza virus on its growth characteristics by using the reverse genetics system.Methods The growth curves of inter-lineage reassortment viruses B/FujianTongan/1565/2013 and B/BeijingHuairou/11764/2013 were determined.The nucleic acids of the two strains were extracted and all gene segments were amplified,then pHW2000 vector was used to construct recombinant plasmids of all segments.The reassortment B/FujianTongan/1565/2013 virus was constructed,then single or different combinations of segments were replaced by segments from B/BeijingHuairou/11764/2013 virus to construct new reassortment viruses.The growth characteristics of all reassortment viruses were analyzed to identify the influence of the gene segments.Results There was significant difference in growth characteristics between the two strains,and the peak value of the growth curve of B/FujianTongan/1565/2013 was about 2 times log higher than that of B/BeijingHuairou/11764/2013.However,there was no significant difference in growth characteristics between B/FujianTongan/1565/2013 and the 8 strains of reassortment viruses with single segment replaced by segment from B/BeijingHuairou/11764/2013,but when the gene combination of HA and NA(surface protein)or PB2,PB1 and PA(polymerase)or PB2,PB1,PA and NP(ribonucleo-protein,RNP)were replaced,the growth characteristics of the reassortment virus decreased significantly.Conclusion The growth characteristics of influenza B virus may be less affected by a single segment,and the synergistic effect of multiple segments is more significant.Therefore,the monitoring of reassortment variant viruses should be strengthened in the surveillance process.
Influenza B virusGene reassortmentReverse geneticsGrowth characteristics
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