Objective To isolate and purify some myxobacteria from Ordos,Inner Mongolia Autonomous Region,detect the inhibitory effect of their metabolites on human glioma cell line U87,and identify the types of the metabolites.Methods Using filter paper induction method,E.coli marking method and rabbit feces induction method,the soil samples of myxo-bacteria collected in Ordos area of Inner Mongolia Autonomous Region were isolated and purified.The purified strains were fermented,the inhibition rate of the fermentation broth on U87 cells was detected by CCK-8 assay,and the metabolites were qualitatively analyzed by chemical chromogenic method.Results Sixteen strains of myxobacteria were isolated and puri-fied,belonging to five genera of myxobacteria.The fermentation supernatant had no inhibitory effect on U87 cells,while the methanol extract of macroporous resin had significant inhibitory effect on U87 cells.Except strain E19,the average highest inhibitory rate of all strains was 80％.There were significant differences in the types of secondary metabolites among different species of strains,among which the metabolites of Corallococcus contained the most species and exhibited the strongest inhibitory effect on the cells,with an inhibition rate of 97.41％.Conclusion The natural products of myxobac-teria can be used as a new anti-glioma drug source for in-depth study,and the related work can provide a reference for the development of new anticancer drugs.
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