Effect of different strains of mice immunized with rabies vaccine on detection of titer
Objective To study the effect of different strains of mice(KM and ICR)immunized with rabies vaccine on the detection of titer.Methods The rabies vaccine and the national standard for the efficacy verification of rabies vaccine for human use(referred to as national standard)were diluted with PBS at the ratios of 1∶25,1∶125,1∶625 and 1∶3 125,and KM and ICR mice with half male and half female were immunized intraperitoneally respectively.Sixteen mice of each strain were immunized with 0.5 mL/mouse at each dilution.The immunization was strengthened once every one week at the same dose and route.The mice in each group were weighed 0,7 and 14 d after the initial immunization.After 14 d of the initial immunization,the mice were subjected to intracranial attack with rabies virus(RABV)CVS2(5-100 LD50),0.03 mL/mouse.The number of mice with death and typical rabies brain symptoms 5 d after the attack was counted.According to the national standard ED50,the relative efficacy was calculated by Reed-Muench method.Results The body mass of the two strains of mice showed an increasing trend during the immunization stage,and the body mass of KM mice increased faster than that of ICR mice.The lgED50 values of the national standard in KM mice were all within the expected range of 2.10-2.75,while the values in ICR mice were higher than the range.The titers of rabies vaccine in KM mice were all significantly lower than those in ICR mice(t=2.887-6.619,each P＜0.05).Conclusion Mouse strains can significantly affect the results of rabies vaccine titer determination,and different standards should be adopted for different strains of mice to ensure the accuracy of vaccine detection results.
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