水痘-带状疱疹病毒Oka-7S株的免疫原性分析
Immunogenicity study of varicella-zoster virus Oka-7S strain
张树杰 1戴龙 1范滢 1周宏达 1房佳 1王梦涵 1徐俊峰 1张夫坤1
作者信息
- 1. 长春祈健生物制品有限公司,吉林长春 130000
- 折叠
摘要
目的 比较水痘-带状庖疹病毒(varicella-zostervirus,VZV)Oka-7S与Oka株免疫原性.方法 用高、中、低(1 000、250、62.5 PFU/剂)剂量VZV Oka-7S及Oka株皮下分别免疫雌性BALB/c小鼠,每组12只,共免疫2次,每次间隔14 d.基于膜抗原荧光抗体试验(fluorescent antibody to membrane antigen assay,FAMA)进行VZV特异性体液免疫评价,酶联免疫斑点(ELISPOT)试验进行VZV特异性细胞免疫评价.结果 VZV Oka-7S和VZV Oka株均可引起特异性体液免疫应答和细胞免疫应答,且具有相当的免疫效果;随免疫次数增加和免疫剂量升高,Oka-7S-2.2组小鼠血清中抗VZV特异性IgG抗体水平均明显升高.结论 VZV Oka-7S株免疫原性与VZV Oka株相当.
Abstract
Objective To compare the immunogenicity of varicella-zoster virus(VZV)Oka-7S strain and VZV Oka strain.Methods Female BALB/c mice were subcutaneously immunized with VZV Oka-7S and Oka strains at high,medium and low doses(1 000,250 and 62.5 PFU/dose)for total two doses at an interval of 14 d,separately,with 12 mice in each group.The VZV-specific humoral immunity was evaluated based on fluorescent antibody to membrane antigen assay(FAMA),and the VZV-specific cellular immunity was evaluated by enzyme-linked immunospot assay(ELISPOT).Results Both VZV Oka-7S and VZV Oka strains induced specific humoral and cellular immune responses,and showed considerable immune effects;The level of anti-VZV specific IgG in serum of mice in Oka-7S-2.2 group increased significantly with the increase of immunization times and immunization doses.Conclusion The VZV Oka-7S strain had comparative immunogenicity with VZV Oka strain.
关键词
水痘-带状疱疹病毒/酶联免疫斑点试验/膜抗原荧光抗体试验/免疫原性Key words
Varicella-zoster virus(VZV)/Enzyme-linked immunospot assay(ELISPOT)/Fluorescent antibody to memb-rane antigen assay(FAMA)/Immunogenicity引用本文复制引用
基金项目
吉林省与中国科学院科技合作高新技术产业化专项(2020SYHZ0010)
出版年
2024
NETL
NSTL
万方数据