重组蛋白药物真核表达系统的研究进展
Research progress on eukaryotic expression system of recombinant protein drugs
张俊河 1杨雯雯 2王天云3
作者信息
- 1. 新乡医学院健康中原研究院,河南新乡 453003;新乡医学院生物化学与分子生物学系,河南新乡 453003;河南省重组药物蛋白表达系统国际联合实验室,河南新乡 453003
- 2. 新乡医学院生物化学与分子生物学系,河南新乡 453003
- 3. 新乡医学院生物化学与分子生物学系,河南新乡 453003;河南省重组药物蛋白表达系统国际联合实验室,河南新乡 453003
- 折叠
摘要
重组蛋白药物具有活性高、特异性强、毒性低等优势,在全球范围内具有广阔的应用前景.目前,用于高效生产重组蛋白药物的表达系统包括原核和真核表达系统.由于真核表达系统具有完善的翻译后修饰和蛋白折叠等优势,在重组蛋白药物生产中得到广泛应用,常用的真核表达系统有酵母、昆虫细胞、哺乳动物细胞和植物细胞表达系统.本文就常见的真核表达系统的特点、应用和发展前景作一综述,以期为重组蛋白药物选择合适的表达系统,促进新型药物研发提供参考.
Abstract
The recombinant protein drugs have the advantages of high activity,high specificity and low toxicity,which have a very broad application prospect in the world.At present,the recombinant therapeutic proteins in vitro are produced from prokaryotic and eukaryotic expression system in order to achieve efficient expression.In view of the advantages such as per-fect post-translational modification and protein folding,eukaryotic expression system has been widely used in the production of recombinant protein drugs.There are four commonly used eukaryotic expression systems,including yeast,insect cell,mammalian cell and plant cell expression systems.In this paper,the characteristics,applications and prospects of several common eukaryotic expression systems are reviewed,in view to providing a reference for the selection of suitable expression systems for recombinant protein drugs and promoting the research and development of new drugs.
关键词
重组蛋白药物/真核表达系统/基因工程Key words
Recombinant protein drugs/Eukaryotic expression system/Gene engineering引用本文复制引用
基金项目
国家自然科学基金(U1604193)
河南省高等学校重点科研项目(23A310015)
出版年
2024
NETL
NSTL
万方数据