Comparison of Coxsackievirus A10 production in Vero cells cultured in different types of bioreactors
Objective To compare the production of Coxsackievirus A10(CV-A10)in Vero cells cultured in WAVE biore-actor,Basket bioreactor and NBS bioreactor.Methods Vero cells were cultured in 2 L WAVE bioreactor,15 L Basket biore-actor and 40 L NBS bioreactor filled with pre-irradiated fibracel disk,fibracel disk and Cytodex 1 microcarriers,respectively,to prepare CV-A10.The adherence efficiency,nutrient metabolites and growth characteristics of Vero cells cultured in diffe-rent types of bioreactors,as well as the virus titers and antigen yields of the prepared CV-A10,were compared.Then,the CV-A10 production process in a 2 L WAVE bioreactor was scaled-up to a 50 L WAVE bioreactor,and the virus titers and antigen yields of CV-A10 prepared in different scale WAVE bioreactors were compared.Results The adherence efficiency of Vero cells on fibracel disk was much higher than that on microcarriers,and the content variation curves of glucose and lactate in different types of bioreactors were consistent.The WAVE bioreactor,Basket bioreactor and NBS bioreactor could all be used for high density culture of Vero cells,while the WAVE bioreactor and Basket bioreactor with fibracel disk could achieve much higher cell density than the NBS bioreactor with Cytodex 1 microcarriers.CV-A 10 could be efficiently pre-pared in different types of bioreactors and there was no significant difference in the virus titers and antigen yields of the prepared CV-A10.Vero cells and CV-A10 were successfully scaled up in the 50 L WAVE bioreactor,and there was no signifi-cant difference in the virus titers and antigen yields of different scale WAVE bioreactors.Conclusion The WAVE biore-actor,Basket bioreactor and NBS bioreactor can all be used for the stable culture of Vero cells and the efficient preparation of CV-A10.
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