2019-2022年长春地区柯萨奇病毒A6的流行病学与分子特征分析
Epidemiology and molecular characteristics analysis of Coxsackievirus A6 in Changchun area from 2019 to 2022
乔凤娟 1孙炳欣 1张兴国 2李晨光 1阴媛 3张舒峰3
作者信息
- 1. 长春市疾病预防控制中心微生物检验所,吉林长春 130119
- 2. 长春市卫健委,吉林长春 130033
- 3. 长春市疾病预防控制中心传染病防治所,吉林长春 130119
- 折叠
摘要
目的 分析2019-2022年长春地区引起手足口病(hand,foot and mouth disease,HFMD)的柯萨奇病毒A6(Cox-sackievirus A6,CV-A6)的流行情况及其VP1区的基因特征,探讨其流行规律,为优化防控策略提供参考.方法 收集2019-2022年长春临床诊断HFMD病例样本878份,采用实时荧光RT-PCR方法进行肠道病毒(enterovirus,EV)核酸检测.对CV-A6阳性样本的VP1基因区进行RT-PCR扩增及测序.再利用MEGA 11构建系统发育树,并使用DNAstarV8.1.3软件分析核苷酸序列同源性和氨基酸变异.结果 878份HFMD病例标本中,检出EV阳性588份,总检出率为66.97％(588/878);检出 CV-A6 阳性245份,CV-A6检出率为41.67％(245/588).125份CV-A6 阳性样本成功扩增并测序了 VP1基因.系统发育分析表明,2019-2022年长春市测定的125株CV-A6毒株均属于D3a基因型,125株CV-A6毒株之间的核苷酸序列同源性为88.1％～100％,与原型株(Gdula)的同源性为81.2％～84.3％.其VP1区氨基酸序列出现了 36个突变位点.结论 2019-2022年长春地区引起HFMD的优势病原体是CV-A6,也是我国大陆地区主流基因亚型D3a,但多条传播链共同循环进化.应进一步加强对CV-A6的监测.
Abstract
Objective To analyse the prevalence of Coxsackievirus A6(CV-A6)causing hand,foot and mouth disease(HFMD)and the genetic characteristics of its VP1 region in Changchun area from 2019 to 2022,explore its epidemic regu-larity,and provide a reference for optimizing the prevention and control strategies.Methods A total of 878 samples of clini-cally diagnosed HFMD cases in Changchun from 2019 to 2022 were collected,and real-time RT-PCR was used for entero-virus(EV)nucleic acid detection.The VP1 gene region of CV-A6-positive samples were amplified by RT-PCR and sequenced.A phylogenetic tree was then constructed using MEGA 11,and DNAstarV8.1.3 software was used to analyze the nucleotide homology and amino acid mutations.Results Among the 878 HFMD samples,588 EV positive were detected,with the total detection rate of 66.97％(588/878);245 CV-A6 positive were detected,with the CV-A6 detection rate of 41.67％(245/588).The VP1 gene was successfully amplified and sequenced from 125 CV-A6 positive samples.Phyloge-netic analysis showed that all 125 CV-A6 strains detected in Changchun from 2019 to 2022 belonged to the D3a genotype.The nucleotide sequence homology among the 125 CV-A6 strains was 88.1％to 100％,and the homology with the prototype strain(Gdula)was 81.2％to 84.3％.There were 36 site mutations in the amino acid sequence of VP 1 region.Conclusion The dominant pathogen causing HFMD in Changchun from 2019 to 2022 was CV-A6,which was also the mainstream gene subtype D3a in the mainland of China.However,multiple transmission chains are circulating and evolving together,suggesting that the surveillance for CV-A6 should be further strengthened.
关键词
手足口病/柯萨奇病毒A6/VP1基因/基因型/分子流行病学Key words
Hand,foot and mouth disease(HFMD)/Coxsackievirus A6(CV-A6)/VP1 gene/Genotype/Molecular epidemi-ology引用本文复制引用
基金项目
吉林省卫生健康科技能力提升项目(2022GW017)
出版年
2024
NETL
NSTL
万方数据