Hepatitis E virus prevalence among blood donors in Wuhan urban agglomeration
Objective To investigate the prevalence of Hepatitis E virus(HEV)among blood donors in Wuhan ur-ban agglomeration,aimed at providing data support for the development of HEV screening strategies for blood donors.Methods A total of 3 329 blood samples were collected from four centralized testing areas(Ezhou,Tianmen,Xiantao and Qianjiang)and screened at Wuhan Blood Center from January to December 2021.Among them,2 737 were eligible blood samples with normal ALT(ALT normal group)and 592 were with elevated ALT(ALT elevated group).Enzyme linked immunosorbent assay(ELISA)was used to detect anti-HEV IgG,anti-HEV IgM and HEV-Ag,and Real-time PCR was used to perform single HEV RNA detection on blood samples with elevated ALT and normal ALT anti-HEV IgM+blood samples.A two-sided Pearson's chi-squared test or Fisher's exact test was used to evaluate the differences in the positive rates of anti-HEV IgG and anti-HEV IgM in different regions and ALT groups.Results The overall posi-tive rates of anti-HEV IgG,anti-HEV IgM,and HEV-Ag in 3 329 blood samples from four regions were 21.63％,1.29％,and 0％,respectively.There was a significant difference in the positive rates of anti-HEV IgG among blood donors in dif-ferent regions(P＜0.05).The highest positive rate of anti-HEV IgG was found in Tianmen 29.44％(136/462),followed by Qianjiang 22.69％(236/1 040),Xiantao 22.66％(230/1 015),and Ezhou 14.53％(118/812).The positive rates of anti-HEV IgG and anti-HEV IgM in blood samples with elevated ALT were significantly higher than that in normal ALT sam-ples(25.68％vs 20.75％,2.53％vs 1.02％,both P＜0.05).However,none of the samples was HEV RNA positive.Conclu-sion HEV is prevalent among blood donors in Wuhan urban agglomeration with extremely low current infection rates,and the prevalence of anti-HEV IgG serum varies among different regions.The prevalence of anti-HEV antibodies in blood donors with elevated ALT is significantly higher than that in normal ALT donors.
Hepatitis E virus(HEV)serological prevalenceblood donornucleic acid screening
万方数据
维普
