Objective To analyze the detection characteristics of a novel serum marker,hepatitis B core-associated antigen(HBcrAg),in the HBsAg-/HBV DNA+blood donors in Wuxi.Methods A total of 37 previous HBsAg-/HBV DNA+blood donors were followed up by telephone and their serum was obtained,and the serum of 22 HBsAg-/HBV DNA+blood donors was detected by electrochemiluminescence and real-time PCR nucleic acid screening as the OBI group for HBcrAg enzyme-linked immunosorbent assay(ELISA).The serum of 20 healthy blood donors who underwent dual ELISA and one nucleic acid testing(NAT)was selected as the healthy control group,and the serum of 20 patients with chronic hepatitis B who were clinically diagnosed by Wuxi Fifth People's Hospital was selected as the experimental CHB group,and HBcrAg ELISA was detected respectively.The correlation analysis between HBcrAg and HBeAb,HB-cAb,ALT and HBV DNA in the OBI group was performed.Results Thirty-seven blood samples were detected by che-miluminescence for HBsAg and NAT,and 22 HBsAg-/HBV DNA+samples were detected in the OBI group,with a de-tection rate of 59.46％.The serum HBcrAg expression content(ng/mL)between the OBI group,the healthy control group and the CHB group were(0.92±0.13),(0.47±0.09)and(1.14±0.23),respectively,and the differences were statisti-cally significant(P＜0.05),the expression of HBcrAg in the OBI group and CHB group was higher than that in the healthy control group(P＜0.05).There was no correlation between HBcrAg and HBeAb,HBcAb,ALT and HBV DNA in-dexes in the OBI group(P＞0.05).Conclusion The expression of HBcrAg in the OBI group and CHB group was high-er than that in the healthy control group,and the serum HBcrAg was not correlated with HBeAb,HBcAb,ALT and HBV DNA to a certain extent.HBcrAg has a good application prospect in screening HBsAg-/HBV DNA+blood donors.
关键词
乙型肝炎核心相关抗原/HBsAg-/HBV/DNA+/献血者/共价闭合环状DNA
Key words
hepatitis B core-related antigen(HBcrAg)/HBsAg-/HBV DNA+/blood donors/covalently closed cir-cular DNA(cccDNA)
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