Objective To investigate the frequency of platelet antibodies in voluntary blood donors and patients in Zhongshan,Guangdong Province,and to study the specificity and cross-matching of platelet antibodies.Methods Platelet antibodies of blood donors and patients were screened by solid-phase immunoadsorption(SPIA),rechecked by flow cytometry(FCM),and antibody specificity was identified by PakPlus enzyme immunoassay,and platelet cross-matching was simulated by SPIA.Results A total of 1 049 blood donor samples and 598 patient samples were tested,with 6(0.57％)and 49(8.19％)samples positive for SPIA,respectively(P＜0.05);In SPIA positive samples,the positive concordance rate of FCM in blood donors and patients was 100％vs 95％,and that of enzyme immunoassay was 100％vs 88％.Among the initial screening positive samples of blood donors,5 were anti-HLA Ⅰ antibodies,accounting for 83％,and 1 was anti CD36 antibody,accounting for 17％,with an incidence rate of 0.10％.Among the 14 samples of en-zyme immunoassay positive patients,2 were anti-GP Ⅱ b/Ⅲa,1 was anti-GP Ⅱ a/Ⅱ a,8 were anti HLA Ⅰ,and 3 were mixed antibodies(HLA Ⅰ,GP Ⅱ b/Ⅲ a,GP Ⅰ a/Ⅱ a).According to the types of antibodies,HLA Ⅰ antibodies were the most common,accounting for 65％(11/17),followed by HPA related anti GP,accounting for 35％(6/17).The majority of patients had a platelet antibody positive typing rate below 30％,accounting for 71.4％(10/14).Conclusions The pos-itive rate of platelet antibody of patients in Zhongshan area is significantly higher than that of voluntary blood donors,and most of them are anti-HLA Ⅰ and anti-GP,and the incidence of anti-CD36 is extremely low.Therefore,it is neces-sary to establish a known platelet antigen donor bank,and at the same time,carry out platelet antibody testing and match-ing of patients,which is helpful to solve the issue of platelet transfusion refractoriness.
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