Effect of long-term frozen plasma on the detection of HBsAg by ELISA
连斯敏 1陈晓华 1林树波 1郭自约 1肖泽斌1
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作者信息
1. 汕头市中心血站,广东汕头 515064
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摘要
目的 评价献血者HBsAg阳性血液标本在-20℃冻存8年后ELISA法HBsAg检测的结果,评估血站目前留样保存方式的有效性.方法 收集本站2014年5月—2015年3月100份经HBsAg ELISA检测阳性的献血者血浆标本,冻存在-20℃冰箱,于2023年解冻标本并通过同种方法再次检测.结果 100份血浆标本的HBsAg再检定性结果均为阳性,再检符合率100％,冻存后S/CO值降低明显(27.52 vs 19.03,P＜0.05).结论 长期冻存会使HB-sAg ELISA检测S/CO值下降,但不影响阳性定性结果.
Abstract
Objective To evaluate the HBsAg detection results of HBsAg+samples after 8 years refrigeration by ELISA and evaluate the effectiveness of the current storage policy of retained samples.Methods A total of 100 HB-sAg+plasma samples by ELISA from May 2014 to March 2015 and refrigerated at-20℃ were collected and retested for HBsAg using the same method after thawing in 2023.Results The HBsAg retest results of 100 plasma samples were all positive,with the concordance rate of 100％,though there was a significant decrease in the S/CO value after refrigera-tion(27.52 vs 19.03,P＜0.05).Conclusion Long-term refrigeration can lead to a decrease in the S/CO value of HBsAg ELISA detection,but it does not affect the positive results.
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