Objective To comprehensively explore the serological characteristics of anti-PP1Pk and potential therapeu-tic strategies for recurrent miscarriage in p-blood type pregnant women.Methods Neutralization with pigeon egg white and human plasma,disruption of IgM antibodies by 2-mercaptoethanol reagent,and complement adding were conducted.Anti-PP1Pk titers under different processing conditions,media and temperatures were determined,and neutralizing effect of hu-man plasma on anti-PP1Pk and its sensitization complement ability were analyzed.Results The titers of anti-PP1Pk in sa-line and column agglutination were 4 and 8,respectively.Low temperature increased titers,while β-mercaptoethanol treat-ment significantly reduced them.Pigeon egg white partially neutralized anti-PP1Pk antibodies.Human plasma was also capa-ble of reducing anti-PP1Pk titers with neutralization capability surpassing that of pigeon egg white,and there were individual differences in neutralization capability.Conclusion The anti-PP1Pk was a blend of antibodies encompassing both IgM and IgG types,exhibiting cold reactivity,and having the potential for complement activation.Human plasma emerges as an ef-fective modulator for diminishing the efficacy of anti-PP1Pk.Plasma transfusion holds promise as a therapeutic avenue for addressing recurrent miscarriages in pregnant individuals with the p phenotype.
关键词
抗-PP1Pk/P1Pk血型系统/Globoside血型系统/鸽子蛋清/中和实验
Key words
anti-PP1Pk/P1Pk blood group system/Globoside blood group system/pigeon egg white/neutralization experiment
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