Objective To identify and analyze a case of ABO discrepancy between forward and reverse blood grouping,and to provide reference for the identification of ambiguous blood group in clinical.Methods ABO and Rh blood group typ-ing,absorption and elution test,and gene sequencing were performed to confirm the ambiguous blood group.Results The sample was identified by absorption and elution test and molecular biological method to be Ael subtype,and was named ABO*AEL.05/ABO*O.01.01 by ISBT.After family investigation,the proband and her second son share the same character-istic mutation site,and was named ABO*AEL.05/ABO*B.01.01 by ISBT.Conclusion Multiple blood group serological tests and molecular biology tests help to identify ABO subtypes,thus assuring the safety,scientificity and rationality of clini-cal blood transfusion.
ABO ambiguous blood group identificationAel subtypeabsorption and elution testpredigree investiga-tionantibody titer
万方数据
维普
