Objective To identify and analyze a case of ABO discrepancy between forward and reverse blood grouping,and to provide reference for the identification of ambiguous blood group in clinical.Methods ABO and Rh blood group typ-ing,absorption and elution test,and gene sequencing were performed to confirm the ambiguous blood group.Results The sample was identified by absorption and elution test and molecular biological method to be Ael subtype,and was named ABO*AEL.05/ABO*O.01.01 by ISBT.After family investigation,the proband and her second son share the same character-istic mutation site,and was named ABO*AEL.05/ABO*B.01.01 by ISBT.Conclusion Multiple blood group serological tests and molecular biology tests help to identify ABO subtypes,thus assuring the safety,scientificity and rationality of clini-cal blood transfusion.
关键词
ABO疑难血型鉴定/Ael亚型/吸收放散试验/家系调查/抗体效价
Key words
ABO ambiguous blood group identification/Ael subtype/absorption and elution test/predigree investiga-tion/antibody titer
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