Blood group serology and gene detection in patients with B(A)subgroup in Jiaozhou,Qingdao
Objective To investigate and analyze the serological and molecular biological characteristics of B(A)sub-group in a tertiary hospital in Jiaozhou,Qingdao.Methods From November 2019 to February 2023,the samples of 12 pa-tients were suspected to be AB subgroup by microcolumn glass bead method and saline test tube method.The exons 6 and 7 of ABO gene were further amplified,sequenced and analyzed to determine the ABO allele type.Results A total of 9 cases of B(A)subgroup were detected in26 065 patients in Jiaozhou,with a detection rate of 0.345‰(9/26 065).Among the 9 cases of B(A)subgroup,8 cases of serological reaction showed Aweak B,and the gene detection was heterozygous for BA.04 gene and O gene.One case of serological reaction showed ABweak,and the gene detection was heterozygous for BA.04 gene and A gene.Conclusion Blood group serological combined with gene detection can accurately identify ABO blood group.B(A)subgroup alleles can exist in individuals with serological reaction of ABweak.
ABO blood groupB(A)subgroupblood group serologygene sequencingQingdao
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