青岛市胶州地区B(A)亚型患者的血型血清学及基因检测分析
Blood group serology and gene detection in patients with B(A)subgroup in Jiaozhou,Qingdao
秦洪伟 1王小霞 2阴瑞兰 2张树超3
作者信息
- 1. 青岛大学基础医学院免疫学系,山东青岛 266071;青岛市胶州中心医院输血科
- 2. 青岛市胶州中心医院输血科
- 3. 青岛大学基础医学院免疫学系,山东青岛 266071;青岛大学附属医院输血科
- 折叠
摘要
目的 调查并分析青岛市胶州地区某三级医院就诊患者B(A)亚型的血清学及分子生物学特征.方法2019 年11 月至2023 年2 月,12 名患者血液标本经微柱玻璃珠法和盐水试管法ABO血型检测疑为AB亚型,进一步对其ABO基因第 6、7 外显子进行直接扩增、测序和分析,确定ABO等位基因型别.结果 青岛市胶州地区 26 065名患者中共检测发现9 例B(A)亚型,检出率为0.345‰(9/26 065);9 例B(A)亚型中,8 例血清学反应表现AwB,基因检测为BA.04 基因与O基因杂合,1 例血清学反应表现为ABw,基因检测为BA.04 基因与A基因杂合.结论 血型血清学结合基因检测可准确鉴定ABO血型,在ABw血清学反应的个体中,有可能存在B(A)亚型等位基因.
Abstract
Objective To investigate and analyze the serological and molecular biological characteristics of B(A)sub-group in a tertiary hospital in Jiaozhou,Qingdao.Methods From November 2019 to February 2023,the samples of 12 pa-tients were suspected to be AB subgroup by microcolumn glass bead method and saline test tube method.The exons 6 and 7 of ABO gene were further amplified,sequenced and analyzed to determine the ABO allele type.Results A total of 9 cases of B(A)subgroup were detected in26 065 patients in Jiaozhou,with a detection rate of 0.345‰(9/26 065).Among the 9 cases of B(A)subgroup,8 cases of serological reaction showed Aweak B,and the gene detection was heterozygous for BA.04 gene and O gene.One case of serological reaction showed ABweak,and the gene detection was heterozygous for BA.04 gene and A gene.Conclusion Blood group serological combined with gene detection can accurately identify ABO blood group.B(A)subgroup alleles can exist in individuals with serological reaction of ABweak.
关键词
ABO血型/B(A)亚型/血型血清学/基因测序/青岛Key words
ABO blood group/B(A)subgroup/blood group serology/gene sequencing/Qingdao引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据