亚洲型DEL母亲产生抗-c致新生儿溶血病的母子输血救治
Hemolytic disease of the newborn caused by anti-c in puerperant of Asian-type DEL treated by transfusion:a case report
姚文娟 1高珺 1穆启明 1舒群峰2
作者信息
- 1. 湖北医药学院附属人民医院,湖北 十堰 442000
- 2. 十堰市中心血站
- 折叠
摘要
目的 探讨 1 例亚洲型DEL母亲产生抗-c引起的新生儿溶血病的母子输血救治方案.方法 盐水试管法鉴定母子ABO血型、Rh表型;经典抗人球蛋白法进行母亲RhD阴性确认、RhD吸收放散试验;经典抗人球蛋白法、抗人球蛋白卡式法和凝聚胺法进行母亲意外抗体筛查及鉴定、抗体效价检测、交叉配血试验;新生儿溶血 3 项试验使用抗人球蛋白卡式法;RHD基因分型采用商品化RHD阴性鉴定基因检测试剂盒(PCR-SSP法)进行产妇RHD基因分型.结果 产妇Rh血清学表型CCee,DEL基因检测结果RHD?1227A,产生抗-c;患儿Rh血清学表型DC-cEe,直抗阳性,红细胞放散阳性,结合母亲孕产史、患儿临床表现和血液检查结果,诊断为抗-c引起的新生儿溶血病.结论 亚洲型DEL母亲产生抗-c并引起新生儿溶血病的患儿选择Rh表型DCCee红细胞输注,母亲选择Rh表型CCee冰冻解冻去甘油红细胞输注,输血有效.
Abstract
Objective To explore a transfusion therapeutic plan for hemolytic disease of the newborn(HDN)caused by anti-c antibodies in mother of Asian-type DEL.Methods The ABO blood type and Rh phenotype of the mother and child were determined using the saline tube method.The mother′s RhD negativity was confirmed through classical anti-human globulin testing and RhD adsorption-elution test.The mother′s unexpected antibodies were screened and their titers were measured using classical anti-human globulin testing,antibody card test and polyamine method.Cross-matching was conduc-ted.The three hemolysis tests were performed by antibody card test.The mother′s RHD gene typing was conducted using a commercially available RHD negative identification gene detection kit(PCR-SSP method).Results The mother exhibited a CCee Rh serological phenotype,and the DEL gene test confirmed the presence of RHD?1227A,indicating the production of anti-c antibodies.The infant displayed a DCcEe Rh serological phenotype,positive for direct antiglobulin test and red cell elution.Based on the mother′s obstetric history,clinical manifestations of the infant and blood examination results,the diag-nosis was HDN caused by anti-c antibodies.Conclusion For infants with HDN caused by anti-c antibodies in mother of A-sian-type DEL,DCCee Rh phenotype red blood cell transfusion is recommended,while CCee Rh phenotype deglycerolized red blood cell transfusion is recommended for the mother.
关键词
抗-c/亚洲型DEL/输血/新生儿溶血Key words
anti-c antibody/Asian-type DEL/blood transfusion/hemolytic disease of the newborn(HDN)引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据