Hemolytic disease of the newborn caused by anti-c in puerperant of Asian-type DEL treated by transfusion:a case report
Objective To explore a transfusion therapeutic plan for hemolytic disease of the newborn(HDN)caused by anti-c antibodies in mother of Asian-type DEL.Methods The ABO blood type and Rh phenotype of the mother and child were determined using the saline tube method.The mother′s RhD negativity was confirmed through classical anti-human globulin testing and RhD adsorption-elution test.The mother′s unexpected antibodies were screened and their titers were measured using classical anti-human globulin testing,antibody card test and polyamine method.Cross-matching was conduc-ted.The three hemolysis tests were performed by antibody card test.The mother′s RHD gene typing was conducted using a commercially available RHD negative identification gene detection kit(PCR-SSP method).Results The mother exhibited a CCee Rh serological phenotype,and the DEL gene test confirmed the presence of RHD?1227A,indicating the production of anti-c antibodies.The infant displayed a DCcEe Rh serological phenotype,positive for direct antiglobulin test and red cell elution.Based on the mother′s obstetric history,clinical manifestations of the infant and blood examination results,the diag-nosis was HDN caused by anti-c antibodies.Conclusion For infants with HDN caused by anti-c antibodies in mother of A-sian-type DEL,DCCee Rh phenotype red blood cell transfusion is recommended,while CCee Rh phenotype deglycerolized red blood cell transfusion is recommended for the mother.
anti-c antibodyAsian-type DELblood transfusionhemolytic disease of the newborn(HDN)
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