Application of anti-idiotypic antibodies in antibody screening and crossmatch tests of patients treated with CD47 monoclonal antibody
Objective To perform pre-transfusion examination and major crossmatch test using CD47 anti-idiotypic an-tibody(CD47 AID)(method 1)and reagent lack of anti-IgG4 anti-human globulin(method 2)in patients treated with CD47 monoclonal antibodies,and evaluate the feasibility of method 1 by comparing the transfusion efficacy of patients after cross matching with two methods.Methods Post-drug samples were collected from 18 clinical subjects treated with CD47 monoclonal antibody in our hospital.Antibody screening and major crossmatch test were performed using method 1 and meth-od 2,and the difference of ΔHb(post-transfusion Hb minus pre-transfusion Hb)was compared after transfusion.The differ-ences in ΔHb after transfusion were analyzed between the test group using method 1 and the control group without CD47 monoclonal antibody using ordinary microcolumn gel method.Results There was no significant difference in ΔHb between the test group using method 1 and test group using method 2(8.40±0.71 vs 7.36±0.94,P>0.05).No significant difference was noticed in ΔHb between the test group using method 1 and the control group without CD47 monoclonal antibody(8.40±0.71 vs 6.59±0.77,P>0.05).Conclusion In the test group,major crossmatch test with method 1 has the same transfusion efficacy as the test with method 2.Method 1 is simple and easy to operate,and the results are objective and accurate.It is recommended to use method 1 for pre-transfusion antibody screening and major crossmatch tests for patients using CD47 monoclonal antibody.
CD47 monoclonal antibodyanti-idiotypic antibodylack of anti-IgG4 anti-human globulin
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