抗独特型抗体在CD47单抗治疗患者抗体筛查和配血中的应用
Application of anti-idiotypic antibodies in antibody screening and crossmatch tests of patients treated with CD47 monoclonal antibody
李鹏 1房阔 1张警丹 1傅炟 1孙佳丽1
作者信息
- 1. 中国医学科学院血液病医院(中国医学科学院血液学研究所) 血液与健康全国重点实验室 国家血液系统疾病临床医学研究中心 细胞生态海河实验室,天津 300020;天津医学健康研究院
- 折叠
摘要
目的 对接受CD47 单克隆抗体治疗的受试组患者使用CD47 抗独特型抗体(CD47 anti-idiotypic antibod-y,CD47 AID)中和法(方法 1)与缺乏抗IgG4 抗人球蛋白法(方法 2)进行输血前抗体筛查和主侧配血,比较经此两种方法配血后的输注效果,以评估CD47 AID中和法的可行性.方法 对本院接受CD47 单克隆抗体治疗的18 位临床受试者用药物后标本,使用方法 1 与方法 2 进行抗体筛查和主侧配血,比较输血后血红蛋白差值ΔHb(输血后Hb-输血前Hb)是否有差异;比较使用方法 1 进行配血的受试组患者与对照组患者(使用普通微柱凝胶法配血且未使用CD47 单抗)输血后ΔHb是否有差异.结果 使用方法1 与使用方法2 进行配血的患者输血后ΔHb比较,两者之间无统计学差异(8.40±0.71 vs 7.36±0.94,P>0.05);使用方法 1 进行配血的受试组患者与未使用CD47 单抗的对照组患者输血后ΔHb比较,两者之间无统计学差异(8.40±0.71 vs 6.59±0.77,P>0.05).结论 受试组患者采用方法 1配血具有与方法 2 配血相同的输注效果,且具有操作简单、结果客观准确的特点,推荐使用方法 1 对使用CD47 单抗的患者进行输血前抗体筛查及主侧配血.
Abstract
Objective To perform pre-transfusion examination and major crossmatch test using CD47 anti-idiotypic an-tibody(CD47 AID)(method 1)and reagent lack of anti-IgG4 anti-human globulin(method 2)in patients treated with CD47 monoclonal antibodies,and evaluate the feasibility of method 1 by comparing the transfusion efficacy of patients after cross matching with two methods.Methods Post-drug samples were collected from 18 clinical subjects treated with CD47 monoclonal antibody in our hospital.Antibody screening and major crossmatch test were performed using method 1 and meth-od 2,and the difference of ΔHb(post-transfusion Hb minus pre-transfusion Hb)was compared after transfusion.The differ-ences in ΔHb after transfusion were analyzed between the test group using method 1 and the control group without CD47 monoclonal antibody using ordinary microcolumn gel method.Results There was no significant difference in ΔHb between the test group using method 1 and test group using method 2(8.40±0.71 vs 7.36±0.94,P>0.05).No significant difference was noticed in ΔHb between the test group using method 1 and the control group without CD47 monoclonal antibody(8.40±0.71 vs 6.59±0.77,P>0.05).Conclusion In the test group,major crossmatch test with method 1 has the same transfusion efficacy as the test with method 2.Method 1 is simple and easy to operate,and the results are objective and accurate.It is recommended to use method 1 for pre-transfusion antibody screening and major crossmatch tests for patients using CD47 monoclonal antibody.
关键词
CD47单克隆抗体/抗独特型抗体/缺乏抗IgG4抗人球蛋白Key words
CD47 monoclonal antibody/anti-idiotypic antibody/lack of anti-IgG4 anti-human globulin引用本文复制引用
基金项目
中国医科院血液安全保障及新产品新技术研究项目(2021-I2M-1-060)
出版年
2024
国家科技期刊平台
NSTL
万方数据