Establishment of internal quality control methodology for blood transfusion compatibility testing
Objective To monitor the effectiveness and accuracy of the blood transfusion compatibility test system by self-made weakly positive internal quality control products.Methods Red blood cells from DAT(-)healthy subjects were selected,and B/RhD(-)E(-)red blood cells were selected as tube 1.A/RhD(+)E(+)was selected as tube 2 to prepare blood group quality control products according to the principle of blood group antigen compatibility,and red blood cell pres-ervation solution and corresponding ABO blood group reagent antibody were added to make the agglutination intensity of mi-crocolumn gel method in reverse blood typing reach a low positive value(1+).Tube 3 and tube 4 were prepared with five different preservation media:plasma,serum,antibody diluent,mixture of equal plasma and antibody diluent,and mixture of equal serum and antibody diluent,respectively.IgM anti-E antibody was added to tube 3,and IgG anti-D antibody was add-ed to tube 4,so that the agglutination intensity of microcolumn gel method reached a low positive value(1+).Results Comparison between the 5 different preservation media showed that the preservation medium of antibody diluent was the most stable for weakly positive antibody(F=11.35,P<0.05),Agglutination intensity 1+is assigned 5 points by AABB Technical Manual,and its score was 5.25±1.75 points.Conclusion The use of self-made weakly positive quality control products can improve the effectiveness,accuracy and sensitivity of the monitoring system,thus achieving internal quality control and en-suring the safety of clinical blood use.
quality control productstransfusion compatibility testingweakly positivedose effectRh phenotype fre-quency
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