合肥地区献血者血液筛查非重复反应性标本与OBI标本间HBV血清学特征分析
HBV serological characteristics between NRR samples and OBI samples of from blood donors in Hefei
李明睿 1王婷 1陈志超 1王婷婷 1何晴1
作者信息
- 1. 安徽省血液中心,安徽 合肥 230031
- 折叠
摘要
目的 了解血液筛查非重复反应性标本与隐匿性乙型肝炎病毒感染(OBI)标本间HBV血清学特征的相关性.方法 收集本科室 2021 年 1 月—2023 年 1 月血液筛查ELISA结果均为阴性,仅NAT反应性的标本共 144份,其中TMA法联检反应性标本92 份,PCR法单人份检测HBV DNA反应性标本52 份.联检反应性标本补充TMA法鉴别检测和PCR法单人份检测,2 种方法检测均无反应性的标本纳入NRR标本组,任1 种方法检出HBV DNA反应性的标本纳入OBI标本组.对 2 组标本完成HBsAg、抗-HBs、HBeAg、抗-HBe、抗-HBc检测,分析NRR标本与OBI标本在血清学模式和阳性率上是否存在差异.结果 联检反应性标本补充检测均阴性标本 53 份,纳入NRR标本组.91 份标本在任 1 种方法中检测出HBV DNA反应性,纳入OBI标本组.2 组标本血清学检测均未检出HBsAg和HBeAg,抗-HBs检出率NRR标本组为 64.15%,OBI标本组为 47.25%;抗-HBc检出率NRR标本组为 86.79%,OBI标本组为 94.51%;抗-HBe检出率NRR标本组为 35.85%,OBI标本组为 52.75%.2 组标本血清学模式:NRR标本组表现最多的模式为抗-HBs+、抗-HBc+(32.08%),OBI标本组表现最多的模式为抗-HBe+、抗-HBc+(37.36%).结论 NRR标本与OBI标本在HBV血清学检测中的部分检测结果间存在差异,但NRR标本中较高的抗-HBc阳性率提示仍有较高的可能存在HBV感染风险.
Abstract
Objective To analyze the correlation of HBV serological characteristics between non-reproducible reactivity(NRR)samples and occult hepatitis B virus infection(OBI)samples for blood screening.Methods A total of 144 sam-ples with negative ELISA(HBV,HCV and HIV test)results and reactive nucleic acid tests(NAT)were collected from Jan-uary 2021 to January 2023 in Anhui Blood Center,including 92 reactive samples by TMA method(combined ID-NAT)and 52 HBV DNA reactive samples by PCR method(ID-NAT).Supplementary differential testing and ID-NAT by PCR were performed on the reactive samples of the combined ID-NAT,samples that were non-reactive by both differential testing and ID-NAT by PCR were included in the NRR group,and samples that were reactive for HBV DNA detected by either method were included in the OBI group.Supplemented with HBsAg,anti-HBs,HBeAg,anti-HBe and anti-HBc tests,the differ-ences in serological pattern and positive rate between NRR samples and OBI samples were analyzed.Results A total of 53 samples were negative for differential testing and ID-NAT and were included in the NRR group,91 samples were detected as HBV DNA reactive in either method and were included in the OBI group.HBsAg and HBeAg were not detected by serologi-cal testing in either group.The detection rates of anti-HBs,anti-HBc and anti-HBe in the NRR group and the OBI group were 64.15%vs 47.25%,86.79%vs 94.51%,35.85%vs 52.75%,respectively.Comparison of serological patterns between the two groups:the most frequent pattern in the NRR group was anti-HBs(+)and anti-HBc(+)(32.08%),and the most frequent pattern in the OBI group was anti-HBe(+)and anti-HBc(+)(37.36%).Conclusion There were differences in some of the test results between the NRR samples and the OBI samples in HBV serological testing,and higher anti-HBc pos-itive rate in the NRR samples suggests a higher possibility of HBV infection.
关键词
乙型肝炎病毒/核酸重复检测非反应性/OBI/HBV血清学阳性Key words
hepatitis B virus/non-reproducible reactivity(NRR)/occult hepatitis B virus infection(OBI)/HBV sero-positive引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据