HBV serological characteristics between NRR samples and OBI samples of from blood donors in Hefei
Objective To analyze the correlation of HBV serological characteristics between non-reproducible reactivity(NRR)samples and occult hepatitis B virus infection(OBI)samples for blood screening.Methods A total of 144 sam-ples with negative ELISA(HBV,HCV and HIV test)results and reactive nucleic acid tests(NAT)were collected from Jan-uary 2021 to January 2023 in Anhui Blood Center,including 92 reactive samples by TMA method(combined ID-NAT)and 52 HBV DNA reactive samples by PCR method(ID-NAT).Supplementary differential testing and ID-NAT by PCR were performed on the reactive samples of the combined ID-NAT,samples that were non-reactive by both differential testing and ID-NAT by PCR were included in the NRR group,and samples that were reactive for HBV DNA detected by either method were included in the OBI group.Supplemented with HBsAg,anti-HBs,HBeAg,anti-HBe and anti-HBc tests,the differ-ences in serological pattern and positive rate between NRR samples and OBI samples were analyzed.Results A total of 53 samples were negative for differential testing and ID-NAT and were included in the NRR group,91 samples were detected as HBV DNA reactive in either method and were included in the OBI group.HBsAg and HBeAg were not detected by serologi-cal testing in either group.The detection rates of anti-HBs,anti-HBc and anti-HBe in the NRR group and the OBI group were 64.15%vs 47.25%,86.79%vs 94.51%,35.85%vs 52.75%,respectively.Comparison of serological patterns between the two groups:the most frequent pattern in the NRR group was anti-HBs(+)and anti-HBc(+)(32.08%),and the most frequent pattern in the OBI group was anti-HBe(+)and anti-HBc(+)(37.36%).Conclusion There were differences in some of the test results between the NRR samples and the OBI samples in HBV serological testing,and higher anti-HBc pos-itive rate in the NRR samples suggests a higher possibility of HBV infection.
hepatitis B virusnon-reproducible reactivity(NRR)occult hepatitis B virus infection(OBI)HBV sero-positive
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