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血液成分二次制备后相关参数变化

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目的 对成分血二次制备后的相关参数变化进行研究,旨在进一步提升血液成分产品质量。方法 选择不同的离心条件用于初次成分血去白细胞悬浮红细胞的制备,并对其进行质量检测,然后以去白细胞悬浮红细胞为二次制备的起始血液,通过ACP215 红细胞处理系统去制备机制洗涤红细胞成分血,并对其进行质量检测,通过对成分血制备参数的变化进行观察研究比较,并从中进行优化调整。结果 不同的离心条件所带来的离心效果相当时,所分离制备的去白细胞悬浮红细胞和冰冻血浆初级血液成分,其容量、血红蛋白、血细胞比积、白细胞残留量等质量控制项目,均符合国家相关标准要求;同时以初次制备的去白细胞悬浮红细胞为起始血液制备的机制,洗涤红细胞二级血液成分,其容量、血红蛋白、上清蛋白含量等质量控制项目,除容量超出标准 7~14 mL外,其余均符合国家相关标准要求,但导致容量超标的原因及采取的解决方法可控制容量达到标准范围,且在红细胞回收率和血浆蛋白清除率上,二者可分别达到 75%和 99%的水平。结论 本实验初次制备与二次制备的血液成分之间有着一定的关联,但血液成分二次制备可视实际情况进行相关参数的灵活调整,以确保制备的成分血产品质量符合国家相关标准要求,以及临床治疗效果和安全。
Changes in related parameters after secondary preparation of blood components
Objective To study the changes in related parameters after secondary preparation of blood components,in order to further improve the quality of blood components.Methods Different centrifugation conditions were selected for the preparation of primary blood component red blood cells in additive solution leukocytes reduced,and the quality was tested.Then,using the red blood cells in additive solution leukocytes reduced as the initial blood for secondary preparation,and the red blood cells were washed through the Haemonetics ACP 215 device,and the quality was tested.The preparation pa-rameters of blood components were observed,compared and optimized.Results Under comparable centrifugation effects of different centrifugation conditions,the quality control items,which of primary blood components of red blood cells in additive solution leukocytes reduced and frozen plasma prepared by the separation,such as volume,hemoglobin,hematocrit and re-sidual white blood cells met the relevant national standards.And the quality control items of secondary blood components of washed red blood cells such as the hemoglobin and superalbumin content both met the relevant national standards,while vol-ume exceeded the standard by 7-14 mL,which can be operated to the standard range.In addition,the recovery rate of red blood cells and the clearance rate of plasma protein could reach 75%and 99%respectively.Conclusion There is a certain correlation between primary and secondary preparation of blood components,but the relevant parameters of secondary prepa-ration of blood components can be flexibly adjusted according to the actual situation to ensure that the quality of prepared blood component products meet the national standards,thus ensuring clinical treatment effect and safety.

blood componentsprimary preparation of blood componentssecondary preparation of blood componentsred blood cells in additive solution leukocytes reducedplasmawashed red blood cells

郭敏、陈君、杨宁、和苗、李建民、杨文娟

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河北省血液中心,河北 石家庄 050071

成分血 初次处理血液成分 二次处理血液成分 去白细胞悬浮红细胞 血浆 洗涤红细胞

河北省医学科学研究课题计划指令性课题

20231233

2024

中国输血杂志
中国输血协会 中国医学科学院输血研究所

中国输血杂志

CSTPCD
影响因子:1.279
ISSN:1004-549X
年,卷(期):2024.37(5)
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