Serological and RHD genotyping characteristics of RhD-negative populations in primary screening in Beijing
Objective To study the RHD genotyping of primary screening RhD negative population in Beijing,and an-alyze the serological characteristics of different genotypes and the distribution of Rh phenotypes.Methods From August 2022 to January 2024,204 RhD negative samples in our hospital were initially screened by microplate or microcolumn agglu-tination method.RhD antigen phenotype was identified by saline tube method,and RhD negative confirmation test was per-formed by microcolumn gel-indirect antiglobulin technology.RHD genotyping was performed by polymerasing chain reaction-sequence specific primmer(PCR-SSP)method and sequencing technology,and the phenotypic frequency and gene frequen-cy were calculated.Results The distribution of Rh serologic phenotypes in 204 RhD negative samples were 112 cases of ccee(54.90%)>73 cases of Ccee(35.78%)>9 cases of CCee(4.41%)>6 cases of ccEe(2.94%)>4 cases of CcEe(1.96%).A total of 7 RHD genotypes were detected in this study,including 129 cases of RHD∗01N.01(63.24%),44 ca-ses of RHD∗01EL.01(21.57%),26 cases of RHD∗01N.03(12.75%),2 cases of RHD∗01N.16(0.98%),1 case of RHD∗06.03.01(0.49%),1 case of weak RHD∗15(0.49%)and 1 case of RHD∗13.01/RHD∗01N.01(0.49%).The frequency and phenotype distribution of Rh blood group antigen gene were consistent with Hardy-Weinberg equilibrium(P>0.05).Conclusion The RHD gene is polymorphic in the primary screening RhD negative population in Beijing,and the most common RhD negative genotype is RHD∗01N.01,and the RHD variant is RHD∗01EL.01.This study may provide data to support the clinical implementation of genotype-matched blood transfusions.
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